Paper-Based Airborne Bacteria Collection and DNA Extraction Kit

Paper-Based Airborne Bacteria Collection and DNA Extraction Kit
The crucial danger from airborne infectious illnesses, bio-weapons, and dangerous micro organism is presently the very best it has ever been in human historical past. The requirement for monitoring airborne pathogens has progressively elevated to defend towards bioterrorism or forestall pandemics, particularly through easy and low-cost platforms which might be utilized in resource-limited settings.
Right here, we developed a paper-based airborne micro organism assortment and DNA extraction equipment appropriate for easy software with minimal devices. Airborne pattern assortment and DNA extraction for PCR evaluation had been built-in within the paper equipment.
We created an easy-to-use paper-based air monitoring system utilizing 3D printing know-how mixed with an air pump. The operation time of your entire course of, comprising air sampling, bacterial cell lysis, purification and focus of DNA, and elution of the DNA analyte, was inside 20 min.
All of the investigations and optimum settings had been examined in a custom-designed closed cupboard system. Within the fabricated cupboard system, the paper equipment operated successfully at a temperature of 25-35 °C and 30-70% relative humidity for air containing 10-106 CFU Staphylococcus aureus. This paper equipment might be utilized for easy, speedy, and cost-effective airborne pathogen monitoring.

New Estimation of Antibiotic Resistance Genes in Sediment Alongside the Haihe River and Bohai Bay in China: A Comparability Between Single and Successive DNA Extraction Strategies

Sediment is considered an important reservoir for antibiotic resistance genes (ARGs). Typically, research describing and evaluating ARGs and their potential hosts in sediment are primarily based on single DNA extractions.
To this point, nonetheless, no examine has been performed to evaluate the affect of DNA extraction effectivity on ARGs in sediment. To find out whether or not the abundance of ARGs is underestimated, we carried out 5 successive extraction cycles with a broadly used industrial equipment in 10 sediment samples collected from the Haihe River and Bohai Bay.
Our outcomes confirmed that gathered DNA yields after 5 extractions had been 1.8-3.1 instances increased than that by single DNA extractions. Excessive-throughput sequencing confirmed that inadequate DNA extraction might generate PCR bias and skew neighborhood construction characterization in sediment.
The relative abundances of some pathogenic micro organism, akin to Enterobacteriales, Lactobacillales, and Streptomycetales, had been considerably totally different between single and successive DNA extraction samples. As well as, real-time fluorescent quantitative PCR (qPCR) confirmed that ARGs, intIextraction cycles.
Among the many measured ARGs, sulfonamide resistance genes and multidrug resistance genes had been dominant subtypes within the examine area. Nonetheless, totally different subtypes of ARGs didn’t reply equally to the extra extraction cycles; some continued to have linear development traits, and a few tended to degree off.
Moreover, extra correlations between ARGs and bacterial communities had been noticed within the successive DNA extraction samples than within the single DNA extraction samples. It’s advised that 3-Four extra extraction cycles are required in future research when extracting DNA from sediment samples.
Taken collectively, our outcomes spotlight that performing successive DNA extractions on sediment samples optimizes the extractable DNA yield and may result in a greater image of the abundance of ARGs and their potential hosts in sediments.

A comparability of various DNA extraction strategies and molecular methods for the detection and identification of foodborne pathogens

Foodborne infections proceed to plague Europe. Meals security monitoring is in disaster as the present methods for detecting pathogens don’t sustain with the worldwide rising of meals manufacturing and consumption. Thus, the event of revolutionary methods for detecting and figuring out pathogenic micro organism has develop into crucial.
The purpose of the current examine was firstly to develop an revolutionary easy and low price methodology of extracting bacterial DNA from contaminated meals and water samples with Salmonella enteric(a) subsp. enteric(a) serovar Typhimurium and Listeria monocytogenes and its comparability with two industrial DNA extraction kits (Qiagen, Macherey-Nagel).
Lastly, pathogens’ detection utilizing two molecular methods (PCR-electrophoresis, LAMP), with the intention to consider one of the best mixture of DNA extraction and identification primarily based on their sensitivity, price, rapidity and ease.
Contemplating the above standards, amongst them, greatest was proved an in-house bacterial DNA extraction methodology, primarily based on the chloroform-isoamyl alcohol protocol, with sure modifications.
This system confirmed statistically comparable outcomes by way of sensitivity, in comparison with the industrial kits, whereas on the similar time maintained excessive rapidity and far decrease price.
Lastly, between the molecular methods, LAMP was discovered extra promising contemplating its simplicity, excessive rapidity and sensitivity. Conclusively, the in-house DNA extraction methodology together with the LAMP approach, was confirmed to be one of the best among the many offered mixtures.
Paper-Based Airborne Bacteria Collection and DNA Extraction Kit

A Easy, Price-Efficient, and Automation-Pleasant Direct PCR Method for Bacterial Group Evaluation

Bacterial communities in water, soil, and people play a necessary position in environmental ecology and human well being. PCR-based amplicon evaluation, akin to 16S rRNA sequencing, is a basic device for quantifying and finding out microbial composition, dynamics, and interactions.
Nevertheless, given the complexity of microbial communities, a considerable variety of samples turns into crucial for analyses that parse the elements that decide microbial composition.
A typical bottleneck in performing these sorts of experiments is genomic DNA (gDNAextraction, which is time-consuming, costly, and infrequently biased primarily based on the kinds of species current. Direct PCR methodology is a probably less complicated and extra correct various to gDNA extraction strategies that don’t require the intervening purification step.
On this examine, we evaluated three variations of direct PCR strategies utilizing numerous heterogeneous bacterial cultures, together with each Gram-positive and Gram-negative species, ZymoBIOMICS microbial neighborhood requirements, and groundwater.
By evaluating direct PCR strategies with DNeasy Blood and Tissue Kits for microbial isolates and DNeasy PowerSoil Kits for microbial communities, we discovered that a particular variant of the direct PCR methodology reveals an general effectivity corresponding to that of the traditional DNeasy PowerSoil protocol within the circumstances we examined.
We additionally discovered that the tactic confirmed increased effectivity for extracting gDNA from the Gram-negative strains in comparison with DNeasy Blood and Tissue protocol. This direct PCR methodology is 1,600 instances inexpensive ($0.34 for 96 samples) and 10 instances less complicated (15 min hands-on time for 96 samples) than the DNeasy PowerSoil protocol.
The direct PCR methodology will also be totally automated and is suitable with small-volume samples, thereby allowing scaling of samples and replicates wanted to help high-throughput large-scale bacterial neighborhood evaluation.
Understanding bacterial interactions and meeting in complicated microbial communities utilizing 16S rRNA sequencing usually requires a big experimental load.
Nevertheless, the present DNA extraction strategies, together with cell disruption and genomic DNA purification, are usually biased, expensive, time-consuming, labor-intensive, and never amenable to miniaturization by droplets or 1,536-well plates as a result of vital DNA loss throughout the purification step for tiny-volume and low-cell-density samples.
A direct PCR methodology might probably clear up these issues. On this examine, we developed a direct PCR methodology which reveals comparable effectivity because the broadly used methodology, the DNeasy PowerSoil protocol, whereas being 1,600 instances inexpensive and 10 instances sooner to execute.

3-min Bacterial Total Protein Extraction Kit

P512L - Ask for price

EZLys? Bacterial Protein Extraction Reagent

8001-100 each
EUR 157.2

EZLys? Bacterial Protein Extraction Reagent

8001-500 each
EUR 352.8

Bacterial Total Protein Extraction Reagent

abx090632-50100assays 50-100 assays
EUR 309.6

Complete Bacterial Protein Extraction Reagent

AR0157 50mL (sufficient for lysis of 10g of bacterial biomass)
EUR 169.2

5X Bacterial Protein Extraction Reagent (Tris)

AKR-180 50mL
EUR 331.2
Description: The Cell Biolabs Bacterial Protein Extraction Reagents contain a gentle, nonionic detergent formulation which quickly extracts functional, recombinant protein from E. coli without mechanical disruption.

5X Bacterial Protein Extraction Reagent (Phosphate)

AKR-181 50mL
EUR 331.2
Description: The Cell Biolabs Bacterial Protein Extraction Reagents contain a gentle, nonionic detergent formulation which quickly extracts functional, recombinant protein from E. coli without mechanical disruption.

Extract-EZ B, Bacterial Protein Extraction Kit, 10X Strength Solution

BS596 1kit, 10prep
EUR 98.63

Genorise® Bacteria DNA Extraction Kit 1000

GR101014 1000
EUR 1045

Genorise® Bacteria DNA Extraction Kit 500

GR101061 500
EUR 480

Genorise® Bacteria DNA Extraction Kit 200

GR101062 200
EUR 258

Genorise® Bacteria DNA Extraction Kit 100

GR101063 100
EUR 144

Bacterial Lipopolysaccharides (LPS) Extraction Kit (up to 50 samples)

1000-100-LPS 1 kit
EUR 489.6

Genorise® Bacteria DNA Extraction Kit 50

GR101064 50
EUR 87

Prolysol BC Protein extraction buffer, Bacteria

P4100-100 100 rxns
EUR 271.2

Exosomal DNA Extraction Kit

P230-25 - Ask for price

Exosomal DNA Extraction Kit

P230-50 - Ask for price

DNA Extraction Kit in Tubes

D100T 1 kit (50 tubes)
EUR 562.8
Description: DNA Extraction Kit in Tubes by Cygnus Technologies is available in Europe via Gentaur.

DNA Extraction Kit in Wells

D100W 1 kit (96 wells plate)
EUR 562.8
Description: DNA Extraction Kit in Wells by Cygnus Technologies is available in Europe via Gentaur.

Nucleon BACC3 Genomic DNA Extraction Kit

EUR 484.8

Bacterial DNA Purification Kit II

K1457-250 250 Preps
EUR 697.2

Bacterial DNA Purification Kit II

K1457-50 50 Preps
EUR 381.6

uPure Urine DNA Extraction Kit

K5011196 1 kit
EUR 999

FFPE Tissue DNA Extraction Kit

K5019100 1 kit
EUR 355

Soil Sample DNA Extraction Kit

SD05 5 preps Ask for price

Soil Sample DNA Extraction Kit

SD25 25 preps
EUR 284.4

xTractPure FFPE DNA Extraction Kit

Z2212002 1 kit (48 extractions)
EUR 399

Small DNA Fragment Extraction Kit

FYG203-100P 100 Preps Ask for price

Small DNA Fragment Extraction Kit

FYG203-300P 300 Preps Ask for price

Large DNA Fragment Extraction Kit

FYG204-100P 100 Preps Ask for price

Large DNA Fragment Extraction Kit

FYG204-300P 300 Preps Ask for price

Bacterial Genomic DNA Isolation Kit

K309-100 each
EUR 451.2

Food DNA Extraction Kit I(50prep)

FDK-1050 50 preps
EUR 328.8

One-Tube Hair DNA Extraction Kit

BS8407 50Preps
EUR 94.72
This easy, cost-effective, and automation-friendly direct-PCR-based 16S rRNA sequencing methodology permits us to review the dynamics, microbial interplay, and meeting of assorted microbial communities in a high-throughput style.


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