ATPase assay kits can be found from a number of suppliers to find out the ATPase exercise of assorted enzymes, resembling membrane transporters, ATP synthases, and a few motor proteins. ATPases catalyze the hydrolysis of a phosphate bond in adenosine triphosphate (ATP) to type adenosine diphosphate (ADP). As ATP hydrolysis yields inorganic phosphate (Pi), the quantity of Pi generated is straight proportional to the exercise of the goal ATPase.
ATPase assays usually measure the change in absorbance by colorimetric detection. ATPase assay kits can be utilized for testing the effectivity of drug uptake, enzyme kinetic research, and learning the results of inhibitors. Go to the ATPase assay equipment suppliers for extra particulars, together with goal enzyme and equipment parts.
Key options and particulars
Assay kind: Enzyme exercise (quantitative)
Detection methodology: Colorimetric
Platform: Microplate reader
Pattern kind: Cell Lysate, Tissue Lysate
Product identify
ATPase Assay Package (Colorimetric)
Detection methodology
Colorimetric
Pattern kind
Cell Lysate, Tissue Lysate
Assay kind
Enzyme exercise (quantitative)
Vary
1 nmol/nicely – 5 nmol/nicely
Species reactivity
Reacts with:Â Mammals, Different species
Product overview
ATPase Assay Package (Colorimetric) (ab234055) offers a fast and straightforward methodology for monitoring ATPase exercise in cell and tissue lysates.
Within the assay, ATPase hydrolyzes ATP releasing ADP and a free phosphate ion, and thru linked reactions, by which phosphate reacts with malachite inexperienced, a powerful, secure chromophore is generated (OD 650 nm).
The assay is easy, delicate, high-throughput adaptable and might detect ATPase Exercise lower than 0.005 U/L.
ATPases catalyzes the decomposition of ATP into ADP and a free phosphate ion. This assay can be utilized within the examine of the various courses of ATPases together with Na+ /Ok+ -ATPase, H + /Ok+ -ATPase, Ca2+ -ATPase, and so forth.
Platform
Microplate reader
atpase exercise assay equipment
Storage directions
Please confer with protocols.
Parts
100 assessments
ATPase Assay Buffer
1 x 25ml
ATPase Developer
1 x 3ml
ATPase Optimistic Management
1 vial
ATPase Substrate
2 vials
Phosphate Commonplace (10 mM)
1 x 0.5ml
DESCRIPTION
Basic description
ATPases and GTPases catalyze the decomposition of ATP or GTP into ADP or GDP and free phosphate. These enzymes play key roles in transport, sign transduction, protein biosynthesis, and cell differentiation.
Options and Advantages
Appropriate with high-throughput dealing with techniques.
Suitability
Appropriate for the detection of ATPase and GTPase exercise and for the screening of ATPase and GTPase inhibitors.
Precept
The ATPase/GTPase Exercise Assay equipment offers a easy and direct process for measuring ATPase/GTPase exercise in a microplate format. This equipment makes use of a single reagent formulation to precisely decide enzyme exercise in 30 minutes at room temperature. The malachite inexperienced reagent varieties a secure darkish inexperienced shade with free phosphate liberated by the enzymes leading to a colorimetric product, measured at 620 nm (600-660 nm), proportional to the enzyme exercise current One unit of exercise is the quantity of enzyme that catalyzes the manufacturing of 1 μmole of free phosphate per minute beneath the assay situations.
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
