ATPase Assay Kits

atpase activity assay kit
ATPase assay kits can be found from a number of suppliers to find out the ATPase exercise of assorted enzymes, resembling membrane transporters, ATP synthases, and a few motor proteins. ATPases catalyze the hydrolysis of a phosphate bond in adenosine triphosphate (ATP) to type adenosine diphosphate (ADP). As ATP hydrolysis yields inorganic phosphate (Pi), the quantity of Pi generated is straight proportional to the exercise of the goal ATPase.
ATPase assays usually measure the change in absorbance by colorimetric detection. ATPase assay kits can be utilized for testing the effectivity of drug uptake, enzyme kinetic research, and learning the results of inhibitors. Go to the ATPase assay equipment suppliers for extra particulars, together with goal enzyme and equipment parts.

Key options and particulars

  • Assay kind: Enzyme exercise (quantitative)
  • Detection methodology: Colorimetric
  • Platform: Microplate reader
  • Pattern kind: Cell Lysate, Tissue Lysate
  • Product identify

    ATPase Assay Package (Colorimetric)
  • Detection methodology

    Colorimetric
  • Pattern kind

    Cell Lysate, Tissue Lysate
  • Assay kind

    Enzyme exercise (quantitative)
  • Vary

    1 nmol/nicely – 5 nmol/nicely
  • Species reactivity

    Reacts with: Mammals, Different species
  • Product overview

    ATPase Assay Package (Colorimetric) (ab234055) offers a fast and straightforward methodology for monitoring ATPase exercise in cell and tissue lysates.

    Within the assay, ATPase hydrolyzes ATP releasing ADP and a free phosphate ion, and thru linked reactions, by which phosphate reacts with malachite inexperienced, a powerful, secure chromophore is generated (OD 650 nm).

    The assay is easy, delicate, high-throughput adaptable and might detect ATPase Exercise lower than 0.005 U/L.

    ATPases catalyzes the decomposition of ATP into ADP and a free phosphate ion. This assay can be utilized within the examine of the various courses of ATPases together with Na+ /Ok+ -ATPase, H + /Ok+ -ATPase, Ca2+ -ATPase, and so forth.

  • Platform

    Microplate reader
atpase activity assay kit
atpase exercise assay equipment

Storage directions

Please confer with protocols.

 

Parts 100 assessments
ATPase Assay Buffer 1 x 25ml
ATPase Developer 1 x 3ml
ATPase Optimistic Management 1 vial
ATPase Substrate 2 vials
Phosphate Commonplace (10 mM) 1 x 0.5ml

DESCRIPTION

Basic description

ATPases and GTPases catalyze the decomposition of ATP or GTP into ADP or GDP and free phosphate. These enzymes play key roles in transport, sign transduction, protein biosynthesis, and cell differentiation.

Options and Advantages

Appropriate with high-throughput dealing with techniques.

Suitability

Appropriate for the detection of ATPase and GTPase exercise and for the screening of ATPase and GTPase inhibitors.

Precept

The ATPase/GTPase Exercise Assay equipment offers a easy and direct process for measuring ATPase/GTPase exercise in a microplate format. This equipment makes use of a single reagent formulation to precisely decide enzyme exercise in 30 minutes at room temperature. The malachite inexperienced reagent varieties a secure darkish inexperienced shade with free phosphate liberated by the enzymes leading to a colorimetric product, measured at 620 nm (600-660 nm), proportional to the enzyme exercise current One unit of exercise is the quantity of enzyme that catalyzes the manufacturing of 1 μmole of free phosphate per minute beneath the assay situations.

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