VITROS SARS CoV2 antigen assay – Procedural Steps: –

Stage 1: Nasopharyngeal swab specimen assortment:
1. Buy a nasopharyngeal swab specimen by inserting the sterile swab into the nostril.
2. Push the sterile swab till resistance is met on the stage of the turbinate.
3. Rotate the sterile swab quite a few occasions in course of the nasopharyngeal wall & go away contained in the place for
10 seconds to saturate the swab tip.
4. Take away the swab from the nostril fastidiously.
5. Place the swab specimen into the viral transport medium buffer tube and shut the tube tightly.
6. Transport the swab pattern in VTM to the laboratory in a chilly chain.
7. The pattern might presumably be saved in Room temperature (Beneath 30◦C) for as loads as 24 hrs from the time of
pattern assortment or at 2 – 8◦C for as loads as 48 hrs from the time of pattern assortment.

SKU:G61RHA20

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H00SIZE:20 Exams/KitSPECIFICITY:98.90%

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Immunochromatography speedy check

Steering to be used of VITROS SARS CoV2 Antigen CLIA primarily based completely Confirm from Ortho Medical

Diagnostics

1. The VITROS SARS CoV2 Antigen assay is a chemiluminescent immunoassay meant for the qualitative
detection of SARS-CoV-2 nucleocapsid antigens in nasopharyngeal (NP) specimens from people who
are suspected of COVID-19 inside one to 5 days of the onset of indicators, or mid-turbinate specimens
collected from asymptomatic people.
2. The assay must be carried out in VITROS 3600 Immunodiagnostics system or VITROS 5600 / VITROS XT 7600
Constructed-in system from Ortho.

Description

Lateral movement immunochromatographic assay that adopted twin coloration system.
For the qualitative detection of SARS-CoV-2 antigen from nasopharyngeal swab specimen.
The check incorporates colloid gold conjugate pad and a membrane strip pre-coated with antibodies particular to SARS-CoV-2 antigen on the check traces (T).
A visual black band (antibody-antigen-antibody gold conjugate refined) seems on the check traces (T) If SARS-CoV-2 antigen is current contained in the specimen.
The administration line (C) reveals that check is carried out appropriately.
Evaluated with panel specimen (n = 75) by PCR
Sensitivity: 90,2 %
Specificity: 100 %

Capabilities

COVID-19, Nasopharyngeal swabs

Calibration Differ

Qualitative

 

Stage 2: Pattern preparation for testing:

1. Pattern preparation need to be carried out in BSL-2 stage cupboard contained in the Laboratory.
2. Combine the swab specimen in VTM tube correctly (vortex roughly 3-5 seconds).
3. Change 100 μL VITROS SARS-CoV-2 Antigen Extraction Buffer correct proper right into a labelled new pattern tube.
4. Add 400 μL viral pattern to the above tube (to take care of 1:Four ratio of extraction buffer: pattern)
5. Combine correctly (Cap/Plug the pattern tube and vortex roughly 3-5 seconds).

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