Concanavalin A (Con A) is an extensively used lectin that selectively binds to the glycoproteins, a-mannopyranosyl and a-glucopyranose. These are generally found all by the cell wall of yeast and fungi, and the cell membrane of mammalian cells and tissues.
Stain the cell wall of yeast and fungi, and the cell membrane of mammalian cells and tissues
Detect glycoconjugates in microscopy and circulation cytometry
Stain glycoproteins in gels
Withstands fixation and permeabilization
Number of 10 CF dyes from UV to near-infrared
Superior CF dyes are good, photostable, and water-soluble
Lectins are moreover versatile probes for detecting glycoconjugates in microscopy and circulation cytometric options and for gel staining of glycoproteins. In neutral and alkaline picks, Con A exists as a tetramer with a molecular weight of roughly 104 kDa. In acidic picks (pH beneath 5.0), Con A exists as a dimer. Con A is more likely to be utilized to selectively stain the cell flooring of dwell cells, and stand as loads as fixation and permeabilization. When cells are mounted and permeabilized forward of staining, fluorescent lectins stain every cell flooring and organelles all by the secretory pathway.
BioMag Plus
Biochem/physiol Actions
Con A should not be blood group particular nonetheless has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for put together. Con A dissociates into dimers at pH 5.6 or beneath. Between pH 5.eight and pH 7.0, Con A exists as a tetramer; above pH 7.Zero bigger aggregates are long-established. Con A reveals mitogenic put together which relies upon its diploma of aggregation. Succinylation results in an brisk dimeric type which stays a dimer above pH 5.6.
Preparation Uncover
Terribly purified by particular affinity chromatography.
Analysis Uncover
Agglutination put together: Determined from serial dilutions of a 1 mg/mL reply (this put together is the underside conc. to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C). Requires Form A Human Blood.
Concanavalin A (Con A) is likely to be going almost definitely in all probability probably the most generally used lectins in cell biology. Our Alexa Fluor 488 conjugate of Con A reveals the acute, inexperienced fluorescence of the Alexa Fluor® 488 dye (absorption/emission maxima ~495/519 nm). Alexa Fluor 488 Con A selectively binds to a-mannopyranosyl and a-glucopyranosyl residues.
Uncover the Proper Stain for Your Utility
Con A and totally completely totally different lectins are carbohydrate binding proteins that acknowledge particular sugar moieties on glycoproteins. The presence and distribution of these targets fluctuate between cell varieties and tissues. In consequence, totally completely totally different cell flooring stains or totally completely totally different lectin conjugates, Wheat Germ Agglutinin (WGA) Conjugates and PNA Lectin Conjugates, would possibly produce greater flooring staining and will also be extra acceptable in your cell type. Lectin conjugates is more likely to be utilized to selectively stain the cell flooring of dwell cells, and stand as loads as fixation and permeabilization.
