BSA Cell Culture Grade Grade 35% BSA Liquid

BSA Cell Culture Grade Grade 35% BSA Liquid
BSA Cell Culture Grade Grade 35% BSA Liquid

 

 

PROPERTIES

biological source

bovine

Quality Level

400

description

endotoxin tested

sterility

sterile-filtered

product line

BioXtra

form

liquid

concentration

35% in DPBS

application(s)

cell culture | mammalian: suitable

pH

6.5-7.5

shipped in

ambient

Related Categories

Albumins and Transport Proteins

DESCRIPTION

General description

BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.

Application

Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.
Select the right albumin for your application with the Albumin Selection Guide

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Abstract

Palmitic acid-modified bovine serum albumin (PAB) was synthetized and found to own remarkable scavenger receptor-A (SR-A) targeting ability in vitro and in vivo, through which activated macrophages took up PAB nanoparticles (PAB NPs) 9.10 times more than bovine serum albumin nanoparticles (BSA NPs) and PAB NPs could delivery anti-inflammatory drugs celastrol (CLT) to inflamed tissues more effectively than BSA NPs. Compared with chondroitin sulfate modified BSA NPs targeting activated macrophages via CD44, PAB NPs show a more prominent targeting effect whether in vivo or in vitro. And PAB also demonstrated excellent biosafety compared to maleylated BSA, a known SR-A ligand that was lethal in our study.

Furthermore, in adjuvant-induced arthritis rats, CLT-PAB NPs significantly improved disease pathology at a lower CLT dose with high safety, compared with CLT-BSA NPs. In addition, compared with the existing ligands with SR-A targeting due to strong electronegativity, the enhanced electronegativity and introduced PA are both important for the SR-A targeting effect of PAB. Therefore, PAB provides a novel direction for the treatment of rheumatoid arthritis and design of new ligands of SR-A.

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Albumin-based Stationary Phases

BSA and HSA are closely related proteins and, consequently, the chromatographic properties of the chiral stationary phases based on these proteins are similar. Sometimes the elution order is reversed between chiral stationary phases based on these proteins; on the HSA-based phases (S)-warfarin elutes before (R)-warfarin, whereas on the BSA-based phases the opposite elution order is observed.

A variety of weakly acidic and neutral compounds are resolved on chiral stationary phases based on BSA and HSA. 2-Arylpropionic acid derivatives such as naproxen, flurbiprofen, ibuprofen, ketoprofen and fenoprofen, reduced folates such as leucovorin and 5-methyltetrahydrofolate, and benzodiazepines such as oxazepam, lorazepam and temazepam are separated. Figure 4 shows enantioseparations of leucovorin, lorazepam hemisuccinate and N-benzoyl-phenylalanine on an HSA-based column. However, cationic compounds are not resolved on BSA and HSA phases. The structure-binding relationship for benzodiazepines using HSA stationary phases reveals that the binding of benzodiazepines occurs at a site that contains both a hydrophobic pocket and an area of cationic charge, and that the chiral recognition occurs in this binding site.

Enantioselectivity of stationary phases based on BSA produced with isolated protein fragments has been investigated. The BSA fragment following peptic digest of BSA has molecular weights of about 35 kDa which is an N-terminal half of amino acid residues 1–307. The BSA fragment phases give longer retentions for benzoin and benzodiazepines, and higher enantioselectivity for lorazepam, benzoin and fenoprofen because of a higher density of chiral recognition site(s), compared with native BSA phases.

shows chromatograms of lorazepam enantiomers on BSA and BSA fragment-based columns. However, it is plausible that the conformation of the BSA fragment might be different from that of the native BSA.

BSA Cell Culture Grade Grade 35% BSA Liquid
BSA Cell Culture Grade Grade 35% BSA Liquid

 Acrosome reaction induced by neoglycoproteins containing Man and GlcNAc

Several BSA-based ‘neoglycoproteins’ were shown to stimulate the acrosome reaction. The binding of Man,Flu-BSA results in a time-dependent receptor aggregation and the induction of acrosome exocytosis in capacitated sperm populations from fertile donors.200 N-acetyl-α-glucosamine (α-GlcNAc) and α-mannose (αMan-BSA) may interact with the putative receptor for one protein of zona pellucida: ZP3 in human spermatozoa.195,202–205 This binding results in the exocytosis of the sperm acrosome (AR).206 The GlcNAc-BSA-induced acrosome reaction is inhibited by a small ligand: N-acetylglucosamine (GlcNAc) and by a purified soluble hydrolase, β-N-acetylglucosaminidase. The induction of the AR with Man-BSA was inhibited by mannose, while soluble α-mannosidase was only partially effective. These data suggest that binding sites for GlcNAc and Man seem to be involved in the induction of the AR in human sperm. The induction of AR in human spermatozoa by GlcNAc-BSA could be used to predict their fertilizing ability in vitro.205

The induction of the acrosome reaction by GlcNAc-BSA and Man-BSA has been shown to involve voltage-dependent Ca2+ channels and a Gi-like guanine regulatory protein.203,207 The GlcNAc-BSA- or Man-BSA-induced AR was completely inhibited by preincubation of spermatozoa with calcium antagonists, indicating a link between the binding of sugar residues of the neoglycoproteins and channel activation. The pretreatment of spermatozoa with Pertussis toxin (PTx) inhibits GlcNAc-BSA- or Man-BSA-induced AR, whereas cholera toxin has no effect. Therefore, the transduction mechanism for GlcNAc-BSA- and Man-BSA-induced AR involves G-proteins of the inhibitory type (GI).204

The effect of ‘peritoneal fluid’ on the human sperm acrosome reaction (AR) was tested.208 When the AR was induced by GlcNAc-BSA, pre-incubation with peritoneal fluid reduced (60%) the percentage of AR, while peritoneal fluid from either the endometriosis group or infertile patients without endometriosis caused no significant differences. These data indicated that peritoneal fluid possesses a protective factor which prevents premature AR.

 

Cell Loading and Sedimentation

The BSA solutions will be transferred into the gradient maker apparatus. This is assembled as in Fig. 12.2 and a linear gradient will be generated from 250 mL of 2% BSA and 250 mL of 4% BSA solutions in the appropriate cell buffer chambers. Next, 50 mL of BSA will be loaded into the loading chamber. A cell suspension will be loaded and the stirrer will start to move under the tube, which makes it possible for the cells to flow through into the sedimentation chamber in about 5–7 min. The gradient valve between the two chambers will be opened to allow the two BSA solutions to mix, and then the release valve will be opened, thus allowing the BSA to flow into the syringe to generate the gradient. After 2.5 h at room temperature, cells will be collected in 12 mL fractions and eluted dropwise into 45 test tubes. Fractions will be placed on ice immediately after collection.

BSA Control for Age-BSA
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COTN15-BSA Alpha Diagnostics 0.5 mg 343.2 EUR
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Dinitrophenyl (DNP)-BSA protein Conjugate
AV-9330-BSA Alpha Diagnostics 10 mg 343.2 EUR
mPEG-BSA (Molecular Weight: 5,000-linear)
PEG-BSA-05K Alpha Diagnostics 100 ug 343.2 EUR
mPEG-BSA (Molecular Weight: 10,000-linear)
PEG-BSA-10K Alpha Diagnostics 100 ug 343.2 EUR
mPEG-BSA (Molecular Weight: 20,000-linear)
PEG-BSA-20K Alpha Diagnostics 100 ug 343.2 EUR
mPEG-BSA (Molecular Weight: 40,000-linear)
PEG-BSA-40K Alpha Diagnostics 100 ug 343.2 EUR
(DRAAGQPAG)3 peptide (repeat-sequence peptide of the P. vivax circumsporozoite protein, CSP) conjugated with BSA
DRAA31-BSA Alpha Diagnostics 0.5 mg 634.8 EUR
(PPPPNAND)3 peptide (repeat-sequence peptide of the P. berghei circumsporozoite protein, CSP) conjugated with BSA
PPPP321-BSA Alpha Diagnostics 0.5 mg 634.8 EUR
(NVDP)4 peptide (minor repeat-sequence peptide of the P. falciparum circumsporozoite protein, CSP) conjugated with BSA
NVDP41-BSA Alpha Diagnostics 0.5 mg 634.8 EUR
(NANP)5 peptide (25-aa, repeat-sequence peptide of the P. falciparum circumsporozoite protein, CSP) conjugated with BSA
NANP51-BSA Alpha Diagnostics 0.5 mg 634.8 EUR
Bovine Serum Albumin (BSA) ELISA Kit
DLR-BSA-Ge-48T DL Develop 48T 464.4 EUR
Bovine Serum Albumin (BSA) ELISA Kit
DLR-BSA-Ge-96T DL Develop 96T 592.8 EUR
Bovine serum albumin (BSA) removal kit (Antibody based aff matrix; sufficient to remove 1-2 mg BSA from Bioprocessed material), 2 ml aff column
800-302-BSA Alpha Diagnostics 1 Kit 416.4 EUR
Bovine serum albumin (BSA) removal kit (Antibody based aff matrix; sufficient to remove 10-20 mg BSA from Bioprocessed material), 10 ml aff column
800-310-BSA Alpha Diagnostics 1 Kit 1074 EUR
Bovine serum albumin (BSA) removal kit (Antibody based aff matrix; sufficient to remove 25-50 mg BSA from Bioprocessed material), 25 ml aff column
800-325-BSA Alpha Diagnostics 1 Kit 1740 EUR
General Bovine Serum Albumin (BSA) ELISA Kit
RDR-BSA-Ge-48Tests Reddot Biotech 48 Tests 661.2 EUR
General Bovine Serum Albumin (BSA) ELISA Kit
RDR-BSA-Ge-96Tests Reddot Biotech 96 Tests 919.2 EUR
General Bovine Serum Albumin (BSA) ELISA Kit
RD-BSA-Ge-48Tests Reddot Biotech 48 Tests 632.4 EUR
General Bovine Serum Albumin (BSA) ELISA Kit
RD-BSA-Ge-96Tests Reddot Biotech 96 Tests 878.4 EUR
Vaccigel Alum adjuvant adsorbed with Bovine Serum Albumin (BSA @1 mg/ml), Vaccine adjuvant
AV-1010-BSA Alpha Diagnostics 1 ml 343.2 EUR
Pre-diluted BSA (bovine serum albumin) standards (1 set) for BCA Optima Protein Assay kit
BCA-BSA-1 Alpha Diagnostics 1 pk 196.8 EUR
Pre-diluted BSA (bovine serum albumin) standards (750 ug/ml) for BCA Optima Protein Assay kit
BCA-BSA-750 Alpha Diagnostics 1 ml 270 EUR
BSA and BGG Stock standards (1 vial each @ 2 mg/ml) for BCA Optima Protein Assay kit
BSA-BGG-1 Alpha Diagnostics 1 pk 343.2 EUR
Pre-diluted BSA (bovine serum albumin) standards (250, 125, 62.5, 31.25, and 0.0 ug/ml) for BCA Optima Protein Assay kit
BCA-BSA-2 Alpha Diagnostics 1 pk 196.8 EUR
Albumin (Human, Mouse, rat, bovine and others) removal kit (synthetic dye based matrix; sufficient to remove 20-40 mg BSA from Bioprocessed material), 2 ml aff column
800-200-BSA Alpha Diagnostics 1 kit 343.2 EUR
Albumin (Human, Mouse, rat, bovine and others) removal kit (synthetic dye based matrix; sufficient to remove 50-100 mg BSA from Bioprocessed material), 5 ml aff column
800-205-BSA Alpha Diagnostics 1 kit 927.6 EUR
Albumin (Human, Mouse, rat, bovine and others) removal kit (synthetic dye based matrix; sufficient to remove 250-500 mg BSA from Bioprocessed material), 25 ml aff column
800-225-BSA Alpha Diagnostics 1 kit 2910 EUR
BSA
31C-CH0114 Fitzgerald 50 mg 159.6 EUR
Ibrutinib drug-Human Serum Albumin Conjugate
IBT16-BSA Alpha Diagnostics 100 ug 634.8 EUR
BSA Control for AGE-BSA
35R-AA007 Fitzgerald 10 mg 268.8 EUR
Testosterone 3 BSA Protein (BSA)
abx060856-1mg Abbexa 1 mg 693.6 EUR
Histamine [G-BSA] (G-G-BSA)
DAG3327 Creative Diagnostics 1mg 1480.8 EUR
Cyclic citrullinated peptide-BSA(CCP-BSA)
E64I00802 EnoGene 100ug 411.6 EUR
HRP-BSA
C050104-10ml Unibiotest 10ml 1336.8 EUR
HRP-BSA
C050104-1ml Unibiotest 1ml 272.4 EUR
FITC-BSA
C050303-10ml Unibiotest 10ml 1336.8 EUR
FITC-BSA
C050303-1ml Unibiotest 1ml 272.4 EUR
BSA Antibody
1-CSB-PA272395 Cusabio
  • 380.40 EUR
  • 292.80 EUR
  • 100ul
  • 50ul
BSA Antibody
1-CSB-PA000390 Cusabio
  • 266.40 EUR
  • 234.00 EUR
  • 100ug
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Acetaminophen [BSA]
DAG2957 Creative Diagnostics 0.5mg 936 EUR
Chloramphenicol [BSA]
DAG2974 Creative Diagnostics 1mg 700.8 EUR
Gentamycin [BSA]
DAG2993 Creative Diagnostics 1mg 858 EUR
PCP [BSA]
DAG3015 Creative Diagnostics 1 mg 858 EUR
estosterone [BSA]
DAG3023 Creative Diagnostics 1mg 1092 EUR
Digoxin [BSA]
DAG3043 Creative Diagnostics 0.5ml 858 EUR
Methamphetamine [BSA]
DAG3101 Creative Diagnostics 1mg 1076.4 EUR
Secobarbital [BSA]
DAG3102 Creative Diagnostics 1 mg 936 EUR

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