BSA Cell Culture Grade Grade 35% BSA Liquid

BSA Cell Culture Grade Grade 35% BSA Liquid
BSA Cell Culture Grade Grade 35% BSA Liquid

 

 

PROPERTIES

biological source

bovine

Quality Level

400

description

endotoxin tested

sterility

sterile-filtered

product line

BioXtra

form

liquid

concentration

35% in DPBS

application(s)

cell culture | mammalian: suitable

pH

6.5-7.5

shipped in

ambient

Related Categories

Albumins and Transport Proteins

DESCRIPTION

General description

BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.

Application

Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.
Select the right albumin for your application with the Albumin Selection Guide

RELATED PRODUCTS

Related Product

Product No.
Description
Pricing
Timestrip Plus 8 °C

Abstract

Palmitic acid-modified bovine serum albumin (PAB) was synthetized and found to own remarkable scavenger receptor-A (SR-A) targeting ability in vitro and in vivo, through which activated macrophages took up PAB nanoparticles (PAB NPs) 9.10 times more than bovine serum albumin nanoparticles (BSA NPs) and PAB NPs could delivery anti-inflammatory drugs celastrol (CLT) to inflamed tissues more effectively than BSA NPs. Compared with chondroitin sulfate modified BSA NPs targeting activated macrophages via CD44, PAB NPs show a more prominent targeting effect whether in vivo or in vitro. And PAB also demonstrated excellent biosafety compared to maleylated BSA, a known SR-A ligand that was lethal in our study.

Furthermore, in adjuvant-induced arthritis rats, CLT-PAB NPs significantly improved disease pathology at a lower CLT dose with high safety, compared with CLT-BSA NPs. In addition, compared with the existing ligands with SR-A targeting due to strong electronegativity, the enhanced electronegativity and introduced PA are both important for the SR-A targeting effect of PAB. Therefore, PAB provides a novel direction for the treatment of rheumatoid arthritis and design of new ligands of SR-A.

More On-  www.store.genprice.com

Albumin-based Stationary Phases

BSA and HSA are closely related proteins and, consequently, the chromatographic properties of the chiral stationary phases based on these proteins are similar. Sometimes the elution order is reversed between chiral stationary phases based on these proteins; on the HSA-based phases (S)-warfarin elutes before (R)-warfarin, whereas on the BSA-based phases the opposite elution order is observed.

A variety of weakly acidic and neutral compounds are resolved on chiral stationary phases based on BSA and HSA. 2-Arylpropionic acid derivatives such as naproxen, flurbiprofen, ibuprofen, ketoprofen and fenoprofen, reduced folates such as leucovorin and 5-methyltetrahydrofolate, and benzodiazepines such as oxazepam, lorazepam and temazepam are separated. Figure 4 shows enantioseparations of leucovorin, lorazepam hemisuccinate and N-benzoyl-phenylalanine on an HSA-based column. However, cationic compounds are not resolved on BSA and HSA phases. The structure-binding relationship for benzodiazepines using HSA stationary phases reveals that the binding of benzodiazepines occurs at a site that contains both a hydrophobic pocket and an area of cationic charge, and that the chiral recognition occurs in this binding site.

Enantioselectivity of stationary phases based on BSA produced with isolated protein fragments has been investigated. The BSA fragment following peptic digest of BSA has molecular weights of about 35 kDa which is an N-terminal half of amino acid residues 1–307. The BSA fragment phases give longer retentions for benzoin and benzodiazepines, and higher enantioselectivity for lorazepam, benzoin and fenoprofen because of a higher density of chiral recognition site(s), compared with native BSA phases.

shows chromatograms of lorazepam enantiomers on BSA and BSA fragment-based columns. However, it is plausible that the conformation of the BSA fragment might be different from that of the native BSA.

BSA Cell Culture Grade Grade 35% BSA Liquid
BSA Cell Culture Grade Grade 35% BSA Liquid

 Acrosome reaction induced by neoglycoproteins containing Man and GlcNAc

Several BSA-based ‘neoglycoproteins’ were shown to stimulate the acrosome reaction. The binding of Man,Flu-BSA results in a time-dependent receptor aggregation and the induction of acrosome exocytosis in capacitated sperm populations from fertile donors.200 N-acetyl-α-glucosamine (α-GlcNAc) and α-mannose (αMan-BSA) may interact with the putative receptor for one protein of zona pellucida: ZP3 in human spermatozoa.195,202–205 This binding results in the exocytosis of the sperm acrosome (AR).206 The GlcNAc-BSA-induced acrosome reaction is inhibited by a small ligand: N-acetylglucosamine (GlcNAc) and by a purified soluble hydrolase, β-N-acetylglucosaminidase. The induction of the AR with Man-BSA was inhibited by mannose, while soluble α-mannosidase was only partially effective. These data suggest that binding sites for GlcNAc and Man seem to be involved in the induction of the AR in human sperm. The induction of AR in human spermatozoa by GlcNAc-BSA could be used to predict their fertilizing ability in vitro.205

The induction of the acrosome reaction by GlcNAc-BSA and Man-BSA has been shown to involve voltage-dependent Ca2+ channels and a Gi-like guanine regulatory protein.203,207 The GlcNAc-BSA- or Man-BSA-induced AR was completely inhibited by preincubation of spermatozoa with calcium antagonists, indicating a link between the binding of sugar residues of the neoglycoproteins and channel activation. The pretreatment of spermatozoa with Pertussis toxin (PTx) inhibits GlcNAc-BSA- or Man-BSA-induced AR, whereas cholera toxin has no effect. Therefore, the transduction mechanism for GlcNAc-BSA- and Man-BSA-induced AR involves G-proteins of the inhibitory type (GI).204

The effect of ‘peritoneal fluid’ on the human sperm acrosome reaction (AR) was tested.208 When the AR was induced by GlcNAc-BSA, pre-incubation with peritoneal fluid reduced (60%) the percentage of AR, while peritoneal fluid from either the endometriosis group or infertile patients without endometriosis caused no significant differences. These data indicated that peritoneal fluid possesses a protective factor which prevents premature AR.

 

Cell Loading and Sedimentation

The BSA solutions will be transferred into the gradient maker apparatus. This is assembled as in Fig. 12.2 and a linear gradient will be generated from 250 mL of 2% BSA and 250 mL of 4% BSA solutions in the appropriate cell buffer chambers. Next, 50 mL of BSA will be loaded into the loading chamber. A cell suspension will be loaded and the stirrer will start to move under the tube, which makes it possible for the cells to flow through into the sedimentation chamber in about 5–7 min. The gradient valve between the two chambers will be opened to allow the two BSA solutions to mix, and then the release valve will be opened, thus allowing the BSA to flow into the syringe to generate the gradient. After 2.5 h at room temperature, cells will be collected in 12 mL fractions and eluted dropwise into 45 test tubes. Fractions will be placed on ice immediately after collection.

BSA
31C-CH0114 Fitzgerald 50 mg 159.6 EUR
Testosterone 3 BSA Protein (BSA)
abx060856-1mg Abbexa 1 mg 693.6 EUR
BSA Control for Age-BSA
2221-BSA Biovision each 210 EUR
BSA Control for AGE-BSA
35R-AA007 Fitzgerald 10 mg 268.8 EUR
THC (BSA)
abx080017-1mg Abbexa 1 mg 510 EUR
SAH (BSA)
abx083009-50ug Abbexa 50 ug 460.8 EUR
PCP [BSA]
DAG3015 Creative Diagnostics 1 mg 858 EUR
THC [BSA]
DAG406 Creative Diagnostics 1mg 858 EUR
Pb2+ [BSA]
DAGA-005B Creative Diagnostics 1mg 1326 EUR
Cd2+ [BSA]
DAGA-006B Creative Diagnostics 1mg 1076.4 EUR
Hg2+ [BSA]
DAGA-007B Creative Diagnostics 1mg 1076.4 EUR
Cu2+ [BSA]
DAGA-008B Creative Diagnostics 1mg 936 EUR
MDMA (BSA)
abx080012-1mg Abbexa 1 mg 444 EUR
EDDP (BSA)
abx080038-1mg Abbexa 1 mg 844.8 EUR
gABA (BSA)
20-abx165727 Abbexa
  • 693.60 EUR
  • 309.60 EUR
  • 2064.00 EUR
  • 828.00 EUR
  • 510.00 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
DHEA [BSA]
DAGPY6646 Creative Diagnostics 500ug 1014 EUR
BSA-SAH
ACT00301-50 Arthus 50µg (20µg) 289.2 EUR
BSA-HCY
ACT00501-100 Arthus 100µg 487.2 EUR
Opium (BSA)
abx080050-1mg Abbexa 1 mg 543.6 EUR
BSA-SAH
E62K00201 EnoGene 100ug 411.6 EUR
Sudan [BSA]
DAGA-075B Creative Diagnostics 1mg 1014 EUR
Biotin [BSA]
DAG3197 Creative Diagnostics 1mg 577.2 EUR
Cocaine (BSA)
abx080052-1mg Abbexa 1 mg 543.6 EUR
Carmine (BSA)
20-abx165660 Abbexa
  • 693.60 EUR
  • 309.60 EUR
  • 2013.60 EUR
  • 811.20 EUR
  • 510.00 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
Estriol (BSA)
20-abx165717 Abbexa
  • 693.60 EUR
  • 309.60 EUR
  • 2064.00 EUR
  • 828.00 EUR
  • 510.00 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
Glycine (BSA)
20-abx165740 Abbexa
  • 727.20 EUR
  • 309.60 EUR
  • 2180.40 EUR
  • 861.60 EUR
  • 526.80 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
Heparin (BSA)
20-abx165746 Abbexa
  • 661.20 EUR
  • 292.80 EUR
  • 1914.00 EUR
  • 777.60 EUR
  • 493.20 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
Digoxin [BSA]
DAG3043 Creative Diagnostics 0.5ml 858 EUR
Glycine [BSA]
DAG3313 Creative Diagnostics 250ug 858 EUR
Glyoxal [BSA]
DAG3314 Creative Diagnostics 250ug 936 EUR
Tylosin [BSA]
DAGA-047B Creative Diagnostics 1mg 1014 EUR
Patulin [BSA]
DAGA-368B Creative Diagnostics 1mg 1076.4 EUR
BSA Antigen
IM112 Cygnus Technologies 1 ml 342 EUR
BSA Antibody
5998-100 Biovision each 424.8 EUR
BSA Antibody
5998-30T Biovision each 175.2 EUR
BSA antibody
70C-CR1016GAP Fitzgerald 1 mg 516 EUR
BSA antibody
70R-12462 Fitzgerald 100 ug 552 EUR
BSA antibody
70-BC67 Fitzgerald 1 mg 229.2 EUR
BSA Antibody
48061-100ul SAB 100ul 399.6 EUR
BSA Antibody
48061-50ul SAB 50ul 286.8 EUR
BSA antibody
10-1911 Fitzgerald 200 ul 651.6 EUR
BSA antibody
10R-A139B Fitzgerald 500 µg 925.2 EUR
BSA antibody
10R-1795 Fitzgerald 100 ul 522 EUR
BSA antibody
10R-10573 Fitzgerald 100 ul 418.8 EUR
BSA antibody
70R-AS001 Fitzgerald 500 ug 236.4 EUR
BSA Antibody
20-abx322792 Abbexa
  • 376.80 EUR
  • 292.80 EUR
  • 100 ug
  • 50 ug
BSA antibody
20R-AR042 Fitzgerald 20 mg 392.4 EUR
BSA antibody
20R-1871 Fitzgerald 100 ug 807.6 EUR
BSA Antibody
35643-100ul SAB 100ul 302.4 EUR
Ketamine (BSA)
abx080008-1mg Abbexa 1 mg 1345.2 EUR

LEAVE A REPLY

Please enter your comment!
Please enter your name here