Capilia TB-Neo assay

capilia tb neo test
capilia tb neo test

Summary

Setting: The power to quickly distinguish between Mycobacterium tuberculosis complicated (MTC) and non-tuberculous mycobacteria (NTM) is essential in scientific follow.

Goal: To judge the usefulness of an immunochromatographic (IC) assay to differentiate between MTC and NTM.

Design: We analysed a panel of 145 cultures from 128 sufferers. The routine molecular identification approaches, such because the AccuProbe™ Mycobacterium tuberculosis complicated tradition identification take a look at and GenoType(®) Mycobacterium assays, have been used as reference strategies.

Outcomes: Of the 101 constructive cultures, 98 have been accurately recognized utilizing the Capilia™ TB-Neo Assay. Of the three discordant isolates, one was recognized as M. bovis bacille Calmette-Guérin (BCG) and two as M. tuberculosis. Though we now have not carried out the sequencing of those strains, some false-negative outcomes have been described resulting from mutations within the mpb64 gene or with some M. bovis BCG strains. We didn’t observe false-positive outcomes or any cross-reaction with 22 NTM strains, 12 non-mycobacterial micro-organisms and 10 unfavorable cultures.

Conclusion: We report good general efficiency (sensitivity 97%, specificity 100%, constructive predictive worth 100% and unfavorable predictive worth 96%) of this fast assay that’s simple to carry out and interpret and doesn’t require pattern preparation, skilled technicians or costly gear.

 

ABSTRACT

The efficiency of the Capilia TB-Neo assay, a new-generation assay, was assessed by figuring out its sensitivity, specificity, reproducibility, and cross-reaction with contaminating organisms. The sensitivity and specificity have been 99.2 and 96.4% and 89.three and 100% in pure and mixed-culture isolates, respectively. The kappa statistic was 95.zero and 77.9% in pure and blended tradition isolates, respectively. There was no cross-reaction with contaminating organisms.

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TEXT

The Capilia TB assay is a fast, easy, and cheap identification take a look at for identification of mycobacterial tradition isolates that has been evaluated extensively (1–7). A brand new technology of this assay, the Capilia TB-Neo assay (Capilia), has been developed, changing the unique assay (Y. Namba, eight September 2010, European patent software EP 2226635 A1). The unique assay has been proven to have sensitivity and specificity starting from 92% to 100% (1, 2, 6). Cross-reaction with different mycobacteria and different micro organism has been demonstrated with the unique assay (1, 7; Y. Namba, eight September 2010, European patent software EP 2226635 A1).

 

hTERT (EF1a, Neo) lentivirus

LVP1131-Neo 1x107 IFU/ml x 200ul
EUR 349
Description: Premade lentivirus expressing human TERT gene under EF1a promoter, containing Neomycin marker.

rtTA (Neo) , Lentiviral particles

LVP400-Neo 1x107 IFU/ml x 200ul
EUR 404
Description: Pre-made lentiviral particles expressing reverse tetracycline Transcriptional Activator (rtTA) under enhanced EF1a promoter with Neomycin antibioic marker, provided in DMEM medium with 10% FBS.

CMV-TetR (Neo) Lentiviral particles 

LVP017-Neo 1x107 IFU/ml x 200ul
EUR 404
Description: Pre-made lentiviral particles expressing tetracycline regulator protein (TetR) under CMV promoter, with Neomycin antibiotic marker.

β-Lactamase (Neo) lentiviral particles

LVP335-Neo 1x107 IFU/ml x 200ul
EUR 349
Description: pre-made lentiviral particles expressing β-Lactamase gene. A Neomycin marker was expressed under RSV promoter. Particles is provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

EF1a-TetR (Neo) Lentiviral particles 

LVP459-Neo 1x107 IFU/ml x 200ul
EUR 404
Description: Pre-made lentiviral particles expressing tetracycline regulator protein (TetR) under enhanced EF1a promoter with Neomycin antibioic marker.

LoxP GFP/RFP ColorSwitch (Neo) lentivirus

LVP460-Neo 1x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles expressing "LoxP-GFP-stop-LoxP-RFP-Stop" cassette under suCMV promoter. It also contains a Neomycin antibiotic selection marker under Rsv promoter. Virus is provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

pWZL-Neo Retroviral Vector

RTV-001-NEO 10 ?g
EUR 566
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

SV40 large T-antigen (Neo), CMV lentiviral particles

LVP016-Neo 1x107 IFU/ml x 200ul
EUR 349
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under suCMV promoter, containing Neomycin marker.

SV40 large T-antigen (Neo), EF1a lentiviral particles

LVP557-Neo 1x107 IFU/ml x 200ul
EUR 349
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under EF1a promoter, containing Neomycin marker.

EF1a Control lentiviral particles (Neo)

EF1a-Null-Neo 1 x107 IFU/ml x 200ul
EUR 349
Description: Negative control lentivirus contains a null spacer insert under EF1a promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the Neomycin marker under RSV promoter.

CMV Control lentiviral particles (Neo)

CMV-Null-Neo 1 x107 IFU/ml x 200ul
EUR 349
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the Neomycin marker under RSV promoter.

hTERT (CMV, Neo) lentivirus in PBS

LVP1130-Neo-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Concentrated lentivirus in PBS, expressing human TERT gene under suCMV promoter, containing Neomycin marker.

hTERT (EF1a, Neo) lentivirus in PBS

LVP1131-Neo-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Concentrated lentivirus in PBS, expressing human TERT gene under EF1a promoter, containing Neomycin marker.

rtTA (Neo) in PBS, Lentiviral particles

LVP400-Neo-PBS 5x107 IFU/ml x 200ul
EUR 507
Description: Pre-made lentiviral particles expressing reverse tetracycline Transcriptional Activator (rtTA) under enhanced EF1a promoter with Neomycin antibioic marker, concentrated particles provided in PBS solution.

Color Switch, CRE report cell line: HEK293-loxP-GFP-RFP Neo)

SC018-Neo 2x10E6 cell/ml x1ml
EUR 4400
Description: CRE reporter cell line (Neo): HEK293 cell line expressing "LoxP-GFP-stop-LoxP-RFP" cassette with Neomycin antibiotic marker

CMV-TetR (Neo) Lentiviral particles  in PBS

LVP017-Neo-PBS 5x107 IFU/ml x 200ul
EUR 507
Description: Pre-made lentiviral particles expressing tetracycline regulator protein (TetR) under CMV promoter, with Neomycin antibiotic marker. Particles pellet was re-suspended in PBS solution.

EF1a-TetR (Neo) Lentiviral particles  in PBS

LVP459-Neo-PBS 5x107 IFU/ml x 200ul
EUR 507
Description: Pre-made lentiviral particles expressing tetracycline regulator protein (TetR) under enhanced EF1a promoter with Neomycin antibioic marker. Particles was pellet down and re-suspended in PBS solution.

LoxP GFP/RFP ColorSwitch (Neo) lentivirus (concentrated)

LVP460-Neo-PBS 5x107 IFU/ml x 200ul
EUR 862
Description: Pre-made lentiviral particles expressing "LoxP-GFP-stop-LoxP-RFP-Stop" cassette under suCMV promoter. It also contains a Neomycin antibiotic selection marker under Rsv promoter. Virus was provied in PBS solution.

TB-16&TB-38

E61H01302 100ug
EUR 343

SV40 large T-antigen (Neo) in PBS, CMV lentiviral particles

LVP016-Neo-PBS 5x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under suCMV promoter, containing Neomycin marker, provided in PBS solution.

SV40 large T-antigen (Neo) in PBS, EF1a lentiviral particles

LVP557-Neo-PBS 5x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under EF1a promoter, containing Neomycin marker, provided in PBS solution.

EF1a Control lentiviral particles (Neo) in PBS

EF1a-Null-Neo-PBS 1 x108 IFU/ml x 200ul
EUR 710
Description: Negative control lentivirus contains a null spacer insert under EF1a promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the Neomycin marker under RSV promoter. The virus was concentrated and provided in PBS solution.

CMV Control lentiviral particles (Neo) in PBS

CMV-Null-Neo-PBS 1 x108 IFU/ml x 200ul
EUR 710
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the Neomycin marker under RSV promoter. The virus was concentrated and provided in PBS solution.

TB-16&TB-38 & ESAT

E61H01303 100ug
EUR 343

TB 21007

B7214-10 10 mg
EUR 324

TB 21007

B7214-50 50 mg
EUR 1211

TB Dry

A2536-1000000 1 kg
EUR 293
Description: TB Dry is a single component high nutrient medium formulatedto maximize yields of recombinant plasmids and cosmids.The most important advantage of TB DRY is that all componentsare combined into a single powder, eliminating the need to autoclave two separate solutions.

TB Dry

A2536-5000000 5 kg
EUR 1194
Description: TB Dry is a single component high nutrient medium formulatedto maximize yields of recombinant plasmids and cosmids.The most important advantage of TB DRY is that all componentsare combined into a single powder, eliminating the need to autoclave two separate solutions.

pEBB-TB

PVT17895 2 ug
EUR 300

Rapid Gold Immuno-assay Kit for Antibody IgG to Tuberculosis (TB)

Z7010010 1 kit
EUR 301
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

TB ESAT antigen

E61H01301 100ug
EUR 382

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