Summary
Setting: The power to quickly distinguish between Mycobacterium tuberculosis complicated (MTC) and non-tuberculous mycobacteria (NTM) is essential in scientific follow.
Goal: To judge the usefulness of an immunochromatographic (IC) assay to differentiate between MTC and NTM.
Design: We analysed a panel of 145 cultures from 128 sufferers. The routine molecular identification approaches, such because the AccuProbe™ Mycobacterium tuberculosis complicated tradition identification take a look at and GenoType(®) Mycobacterium assays, have been used as reference strategies.
Outcomes: Of the 101 constructive cultures, 98 have been accurately recognized utilizing the Capilia™ TB-Neo Assay. Of the three discordant isolates, one was recognized as M. bovis bacille Calmette-Guérin (BCG) and two as M. tuberculosis. Though we now have not carried out the sequencing of those strains, some false-negative outcomes have been described resulting from mutations within the mpb64 gene or with some M. bovis BCG strains. We didn’t observe false-positive outcomes or any cross-reaction with 22 NTM strains, 12 non-mycobacterial micro-organisms and 10 unfavorable cultures.
Conclusion: We report good general efficiency (sensitivity 97%, specificity 100%, constructive predictive worth 100% and unfavorable predictive worth 96%) of this fast assay that’s simple to carry out and interpret and doesn’t require pattern preparation, skilled technicians or costly gear.
ABSTRACT
The efficiency of the Capilia TB-Neo assay, a new-generation assay, was assessed by figuring out its sensitivity, specificity, reproducibility, and cross-reaction with contaminating organisms. The sensitivity and specificity have been 99.2 and 96.4% and 89.three and 100% in pure and mixed-culture isolates, respectively. The kappa statistic was 95.zero and 77.9% in pure and blended tradition isolates, respectively. There was no cross-reaction with contaminating organisms.
TEXT
The Capilia TB assay is a fast, easy, and cheap identification take a look at for identification of mycobacterial tradition isolates that has been evaluated extensively (1–7). A brand new technology of this assay, the Capilia TB-Neo assay (Capilia), has been developed, changing the unique assay (Y. Namba, eight September 2010, European patent software EP 2226635 A1). The unique assay has been proven to have sensitivity and specificity starting from 92% to 100% (1, 2, 6). Cross-reaction with different mycobacteria and different micro organism has been demonstrated with the unique assay (1, 7; Y. Namba, eight September 2010, European patent software EP 2226635 A1).
CMV-TetR (Neo) Lentiviral particles |
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| LVP017-Neo | GenTarget | 1x107 IFU/ml x 200ul | EUR 484.8 |
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Description: Pre-made lentiviral particles expressing tetracycline regulator protein (TetR) under CMV promoter, with Neomycin antibiotic marker. |
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β-Lactamase (Neo) lentiviral particles |
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| LVP335-Neo | GenTarget | 1x107 IFU/ml x 200ul | EUR 418.8 |
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Description: pre-made lentiviral particles expressing β-Lactamase gene. A Neomycin marker was expressed under RSV promoter. Particles is provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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EF1a-TetR (Neo) Lentiviral particles |
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| LVP459-Neo | GenTarget | 1x107 IFU/ml x 200ul | EUR 484.8 |
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Description: Pre-made lentiviral particles expressing tetracycline regulator protein (TetR) under enhanced EF1a promoter with Neomycin antibioic marker. |
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LoxP GFP/RFP ColorSwitch (Neo) lentivirus |
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| LVP460-Neo | GenTarget | 1x107 IFU/ml x 200ul | EUR 852 |
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Description: Pre-made lentiviral particles expressing "LoxP-GFP-stop-LoxP-RFP-Stop" cassette under suCMV promoter. It also contains a Neomycin antibiotic selection marker under Rsv promoter. Virus is provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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pWZL-Neo Retroviral Vector |
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| RTV-001-NEO | Cell Biolabs | 10 ?g | EUR 679.2 |
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Description: Use this construct to clone your gene for downstream recombinant retroviral packaging |
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SV40 large T-antigen (Neo), CMV lentiviral particles |
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| LVP016-Neo | GenTarget | 1x107 IFU/ml x 200ul | EUR 418.8 |
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Description: Pre-made lentiviral particles expressing SV40 large T-antigen under suCMV promoter, containing Neomycin marker. |
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SV40 large T-antigen (Neo), EF1a lentiviral particles |
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| LVP557-Neo | GenTarget | 1x107 IFU/ml x 200ul | EUR 418.8 |
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Description: Pre-made lentiviral particles expressing SV40 large T-antigen under EF1a promoter, containing Neomycin marker. |
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CMV Control lentiviral particles (Neo) |
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| CMV-Null-Neo | GenTarget | 1 x107 IFU/ml x 200ul | EUR 418.8 |
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Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the Neomycin marker under RSV promoter. |
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EF1a Control lentiviral particles (Neo) |
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| EF1a-Null-Neo | GenTarget | 1 x107 IFU/ml x 200ul | EUR 418.8 |
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Description: Negative control lentivirus contains a null spacer insert under EF1a promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the Neomycin marker under RSV promoter. |
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hTERT (CMV, Neo) lentivirus in PBS |
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| LVP1130-Neo-PBS | GenTarget | 1x108 IFU/ml x 200ul | EUR 852 |
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Description: Concentrated lentivirus in PBS, expressing human TERT gene under suCMV promoter, containing Neomycin marker. |
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hTERT (EF1a, Neo) lentivirus in PBS |
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| LVP1131-Neo-PBS | GenTarget | 1x108 IFU/ml x 200ul | EUR 852 |
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Description: Concentrated lentivirus in PBS, expressing human TERT gene under EF1a promoter, containing Neomycin marker. |
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rtTA (Neo) in PBS, Lentiviral particles |
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| LVP400-Neo-PBS | GenTarget | 5x107 IFU/ml x 200ul | EUR 608.4 |
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Description: Pre-made lentiviral particles expressing reverse tetracycline Transcriptional Activator (rtTA) under enhanced EF1a promoter with Neomycin antibioic marker, concentrated particles provided in PBS solution. |
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Color Switch, CRE report cell line: HEK293-loxP-GFP-RFP Neo) |
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| SC018-Neo | GenTarget | 2x10E6 cell/ml x1ml | EUR 5280 |
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Description: CRE reporter cell line (Neo): HEK293 cell line expressing "LoxP-GFP-stop-LoxP-RFP" cassette with Neomycin antibiotic marker |
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CMV-TetR (Neo) Lentiviral particles in PBS |
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| LVP017-Neo-PBS | GenTarget | 5x107 IFU/ml x 200ul | EUR 608.4 |
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Description: Pre-made lentiviral particles expressing tetracycline regulator protein (TetR) under CMV promoter, with Neomycin antibiotic marker. Particles pellet was re-suspended in PBS solution. |
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EF1a-TetR (Neo) Lentiviral particles in PBS |
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| LVP459-Neo-PBS | GenTarget | 5x107 IFU/ml x 200ul | EUR 608.4 |
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Description: Pre-made lentiviral particles expressing tetracycline regulator protein (TetR) under enhanced EF1a promoter with Neomycin antibioic marker. Particles was pellet down and re-suspended in PBS solution. |
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LoxP GFP/RFP ColorSwitch (Neo) lentivirus (concentrated) |
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| LVP460-Neo-PBS | GenTarget | 5x107 IFU/ml x 200ul | EUR 1034.4 |
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Description: Pre-made lentiviral particles expressing "LoxP-GFP-stop-LoxP-RFP-Stop" cassette under suCMV promoter. It also contains a Neomycin antibiotic selection marker under Rsv promoter. Virus was provied in PBS solution. |
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TB-16&TB-38 |
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| E61H01302 | EnoGene | 100ug | EUR 411.6 |
SV40 large T-antigen (Neo) in PBS, CMV lentiviral particles |
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| LVP016-Neo-PBS | GenTarget | 5x107 IFU/ml x 200ul | EUR 852 |
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Description: Pre-made lentiviral particles expressing SV40 large T-antigen under suCMV promoter, containing Neomycin marker, provided in PBS solution. |
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SV40 large T-antigen (Neo) in PBS, EF1a lentiviral particles |
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| LVP557-Neo-PBS | GenTarget | 5x107 IFU/ml x 200ul | EUR 852 |
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Description: Pre-made lentiviral particles expressing SV40 large T-antigen under EF1a promoter, containing Neomycin marker, provided in PBS solution. |
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CMV Control lentiviral particles (Neo) in PBS |
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| CMV-Null-Neo-PBS | GenTarget | 1 x108 IFU/ml x 200ul | EUR 852 |
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Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the Neomycin marker under RSV promoter. The virus was concentrated and provided in PBS solution. |
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EF1a Control lentiviral particles (Neo) in PBS |
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| EF1a-Null-Neo-PBS | GenTarget | 1 x108 IFU/ml x 200ul | EUR 852 |
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Description: Negative control lentivirus contains a null spacer insert under EF1a promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the Neomycin marker under RSV promoter. The virus was concentrated and provided in PBS solution. |
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TB-16&TB-38 & ESAT |
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| E61H01303 | EnoGene | 100ug | EUR 411.6 |
TB Dry |
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| A2536-1000000 | ApexBio | 1 kg | EUR 351.6 |
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Description: TB Dry is a single component high nutrient medium formulatedto maximize yields of recombinant plasmids and cosmids.The most important advantage of TB DRY is that all componentsare combined into a single powder, eliminating the need to autoclave two separate solutions. |
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TB Dry |
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| A2536-5000000 | ApexBio | 5 kg | EUR 1432.8 |
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Description: TB Dry is a single component high nutrient medium formulatedto maximize yields of recombinant plasmids and cosmids.The most important advantage of TB DRY is that all componentsare combined into a single powder, eliminating the need to autoclave two separate solutions. |
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TB 21007 |
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| B7214-10 | ApexBio | 10 mg | EUR 388.8 |
TB 21007 |
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| B7214-50 | ApexBio | 50 mg | EUR 1453.2 |
pEBB-TB |
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| PVT17895 | Lifescience Market | 2 ug | EUR 360 |
TB ESAT antigen |
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| E61H01301 | EnoGene | 100ug | EUR 458.4 |
TB CFP10 Antigen |
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| E61H01304 | EnoGene | 100ug | EUR 411.6 |
Rat Triglycerides（TB） |
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| QY-E11920 | Qayee Biotechnology | 96T | EUR 448.8 |
Terbium (Tb) Donor |
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| 60394 | BPS Bioscience | 30 µl | EUR 190 |
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Description: The Terbium Donor, also known as Tb Donor, is used for phosphodiesterase (PDE) TR-FRET (Time Resolved Fluorescence Resonance Energy Transfer) technology based assays including PDE1B, PDE1C, PDE3A, PDE4D, and PDE10A. In these assays, terbium conjugates with phosphate. _x000D_ |
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