The Aviva Systems Biology CFB ELISA Kit (Human) (OKEH01427) is based on standard sandwich enzyme-linked immunosorbent assay technology. A CFB-specific antibody has been precoated on a 96-well plate (12 x 8-well strips) and blocked. Standards or test samples are added to the wells, incubated, and removed. Add a biotinylated detector antibody specific for CFB, incubate, and wash.
The avidin-peroxidase conjugate is then added, incubated, and the unbound conjugate is washed. An enzymatic reaction occurs by adding TMB substrate that is catalyzed by HRP generating a blue product that changes to yellow after adding the acid stop solution. The density of the yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CFB captured in the well.
Reconstitution and Storage Store as indicated in the product manual.
Sensitivity Serum, Plasma, tissue homogenates, Cell culture supernates, Other biological fluids
Specificity 0.297 ng/mL
Assay Info Assay Methodology: Quantitative Sandwich ELISA
ELISA Kit Component
|CFB Microplate||96 Wells (12 x 8 Well strips)|
|CFB Lyophilized Standard||2|
|100X Biotinylated CFB Detector Antibody||1 x 120 uL|
|100X Avidin-HRP Conjugate||1 x 120 uL|
|Sample Diluent||1 x 20 mL|
|Detector Antibody Diluent||1 x 12 mL|
|Conjugate Diluent||1 x 12 mL|
|25X Wash Buffer||1 x 30 mL|
|TMB Substrate||1 x 10 mL|
|Stop Solution||1 x 10 mL|
Description of Target
Factor B, which is part of the alternative pathway of the complement system, is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate C3 or C5 convertase.
It has also been implicated in the proliferation and differentiation of preactivated B lymphocytes, the rapid spread of peripheral blood monocytes, the stimulation of lymphocyte blastogenesis, and the lysis of erythrocytes. Ba inhibits the proliferation of pre-activated B lymphocytes.