CHO HCP (Host Cell Protein) ELISA Kit
Detection method: colorimetric
Essay type: sandwich (quantitative)
Distance: 3.3ng/mL – 810ng/mL
Rehearsal time: 3h 0m
Test duration: Standard multi-step assay
The CHO HCP ELISA (Host Cell Protein) Kit (ab240996) is designed to quantitatively measure host cell protein (HCP) contamination in bulk products expressed in Chinese Hamster Ovary (CHO) expression systems.
The 96-well plate provided has been previously coated with rabbit anti-CHO HCP antibodies to capture CHO proteins from biological samples. After incubation to allow capture of the CHO protein by the antibodies on the plate, a second anti-CHO HCP antibody, conjugated with biotin, is added and incubated to allow it to bind to the captured CHO proteins. After incubation, the plate is washed and a streptavidin-HRP conjugate is added and incubated for 30 min.
The streptavidin-HRP conjugate will be captured by any biotinylated antibody bound to the plate. After a wash step to remove unbound conjugate, TMB substrate is added and converted to a coloured product by captured HRP in proportion to the amount of HCP bound to the plate. After a brief incubation to allow colour development, the reaction is stopped and the intensity of the colour generated is detected on a spectrophotometer plate reader capable of measuring a wavelength of 450nm.
Platform: Precoated microplate (12 x 8-well strips)
Store at +4°C. Check the protocols.
The Chinese hamster ovary cell line is used for antibody production and recombinant protein expression methods.
Chinese hamster ovary cell line