coomassie staining solution

coomassie staining solution
coomassie staining solution

Technical data: 

Coomassie R-250 and G-250 dyes are two most typical chemical types of Coomassie dyes, as disulfonated
triphenylmethane compounds. The R-250 (red-tinted) lacks two methyl teams which might be current within the G-250 (greentinted) type. The “250” initially denoted the purity of the dye.
The totally different colors are a results of the totally different charged states of the dye molecule.
At a pH of lower than Zero the G250 dye caries all three nitrogen atoms with a optimistic cost, it has a purple color with an
absorption most at a wavelength of 465 nm. At a pH of round 1 the dye bears an general cost of +1 (the two
sulfonic acid teams are negatively cost, due to theirextremely low pKa), so the dye is inexperienced with an absorption
most at 620 nm whereas above pH 2 the dye is brilliant blue with a most at 595 nm. At pH 7 the nitrogen atom of the diphenylamine moiety carries a optimistic cost, with an general cost of −1, and the dye has an extinction
coefficient of 43 000 M−1 cm−1
.
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The dye interacts electrostatically with the amino and carboxyl teams of proteins (noncovalently), so from primarily fundamental
aminoacids (arginine principally, lysine, histidine). Hydrophobic interactions are additionally considerably concerned. (phenylalanine,
tyrosine, tryptophane).
When dissolved in 0.01M citrate buffer at pH 3.0, Coomassie has an absorption most at 555nm; protein-dye
complicated is characterised by a peak barely broader than that of the free dye with a most at 549nm. Therefore, certain
and unbound dye will be distinguished (precept of Bradford assay).
Sometimes Coomassie gel stains and protein Bradford-type assay reagents are formulated as very acidic options in 25 to
50% methanol. In acidic situations, the dye binds to proteins primarily by fundamental amino acids (primarily arginine, lysine and histidine), whereas the formation of the complicated stabilizes the negatively charged anionic type of the dye producing the blue color. The variety of Coomassie dye ligands certain to every protein molecule is roughly proportional to the variety of optimistic fees discovered on the protein. Protein-binding causes the dye to alter from reddish-brown to brilliant blue (absorption most equals 595nm).

The aqueous-solubility of the G-250 dye is taken to good account in protocols of colloidal Coomassie staining.
There’s an interference from SDS detergent, particularly with G250 dye (see various BC protein Assay for assaying
proteins with SDS).

coomassie staining solution
coomassie staining resolution

Coomassie Sensible Blue staining resolution

Dissolve 1 g of Coomassie Sensible Blue (Bio-Rad) in 1 liter of the next resolution:

Methanol (50% [v/v])

Glacial acetic acid (10% [v/v])

H2O (40%)

Stir the answer for 3-Four hours after which filter by Whatman filter paper. Retailer at room temperature.

 

Reagent Amount (for 100 mL) Ultimate focus
Coomassie Sensible Blue R-250  0.05 g   0.05%
Methanol  50 mL 50% (v/v)
Glacial acetic acid  10 mL 10% (v/v)
H2O to 100 mL
Dissolve the Coomassie Sensible Blue R-250 dye, after which filter by a Whatman No. 1 filter to take away any particulate matter. May be saved at room temperature.

 

Coomassie Brilliant Blue Gel Staining Kit

abx090653-1Kit 1 Kit
EUR 189
  • Shipped within 5-10 working days.

SDS-Blue™ - Coomassie based solution for protein staining in SDS-PAGE

04-GSB
  • EUR 120.00
  • EUR 80.00
  • 1L
  • 500mL


  • Working procedure:
    1) Invert the bottle a few times to ensure the solution is properly suspended.
    2) Take the gel out of the gel tank and submerse the gel in enough SDS-Blue to cover the gel.
    3) Bands will be visi
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Description: SDS-Blue™ is an innovative patented formula, based on Coomassie blue, that comes in a convenient ready to use format for staining proteins in SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis). The formulation of SDS-Blue™ provides numerous advantages compared to the classic Coomassie staining or to other similar protein stains. SDS-Blue™ provides higher sensitivity, virtually no background and eliminates the need for destaining of the gel due to its high specificity and affinity to bind to protein only. Not only does SDS-Blue™ yield clear and sharp bands, but it also contains no methanol and acetic acid, making it non-hazardous, safe to handle and friendly to the environment when disposed of. Two other advantages that make SDS-Blue™ the better option is that it is not light sensitive and can be stored at ambient temperature for 24 months. And this provides a considerable convenience, especially to laboratories that need and keep big amount of protein staining solutions – no more jammed refrigerators, you can keep SDS-Blue™ wherever it is most convenient for You!

Ponceau S Staining Solution

B7778-100000 100 g
EUR 163

Ponceau S Staining Solution

B7778-50000 50 g
EUR 126

ProsiBlue Gel Staining Solution

P7300-050 500ml
EUR 174

Ponceau S Staining Solution

PW005 100ml
EUR 69.14
  • Product category: Biochemicals/Indicators/Stains/Peptide/Protein Related

7-AAD Viability Staining Solution

EXB0026 400 tests
EUR 76

SafePinky DNA Gel Staining Solution

S1000-050 500ml
EUR 131

SafePinky DNA Gel Staining Solution

S1000-100 2X500ml
EUR 188

SafePinky DNA Gel Staining Solution

S1000-200 4X500ml
EUR 280

SuperPower (TM) Stain Tablet, Fast Staining Coomassie G-250

DG-STAIN 5drytablets, 5prep
EUR 160.2
  • Product category: Molecular Biology Kits/Protein - Staining

Protein Stain-EZG Rapid Coomassie Blue Stain Solution

BSP041 1KIT, 10prep
EUR 76.1
  • Product category: Molecular Biology Kits/Protein - Staining

dsGreen Gel Staining Solution, 10000×, 1 mL

20010 1 mL
EUR 134

dsGreen Gel Staining Solution, 10000×, 0.5 mL

10010 0.5 mL
EUR 89

Protein Stain-EZ A Ultra Sensitive Coomassie Blue Stain Solution

SK3011 10Minigel, 10UNIT
EUR 66.53
  • Product category: Biochemicals/Indicators/Stains/Peptide/Protein Related

SafePinky DNA Gel Staining Solution(10,000X) In Water

S1001-025 250ul
EUR 161

SafePinky DNA Gel Staining Solution(10,000X) In Water

S1001-050 2x250ul
EUR 247

SafePinky DNA Gel Staining Solution(10,000X) In Water

S1001-100 1ml
EUR 435

SafePinky DNA Gel Staining Solution(10,000X) In Water

S1001-500 5x1ml
EUR 1643

Safe E-Red DNA Gel Staining Solution(1000x)

S1002-001 1ml
EUR 111

Safe E-Red DNA Gel Staining Solution(1000x)

S1002-005 5x1ml
EUR 226

Safe E-Red DNA Gel Staining Solution(1000x)

S1002-010 10ml
EUR 300

Safe E-Red DNA Gel Staining Solution(1000x)

S1002-020 20ml
EUR 496

Coomassie Brilliant Blue G250

abx090654-5g 5 g
EUR 189
  • Shipped within 5-10 working days.

Coomassie Brilliant Blue R250

abx090655-5g 5 g
EUR 189
  • Shipped within 5-10 working days.

Coomassie (Bradford) Protein Assay Kit

AR0145 1kit
EUR 70

Coomassie brilliant blue R-250

20-abx082400
  • EUR 230.00
  • EUR 189.00
  • 25 g
  • 5 g
  • Shipped within 5-10 working days.

Coomassie brilliant blue G-250

20-abx082402
  • EUR 230.00
  • EUR 189.00
  • 25 g
  • 5 g
  • Shipped within 5-10 working days.

Coomassie brilliant blue R-250

20-abx082561
  • EUR 217.00
  • EUR 175.00
  • 25 g
  • 5 g
  • Shipped within 5-10 working days.

Coomassie brilliant blue G-250

20-abx082562
  • EUR 217.00
  • EUR 175.00
  • 25 g
  • 5 g
  • Shipped within 5-10 working days.

Coomassie Brilliant Blue R-250

CH026 50 g
EUR 175

10G Coomassie Blue R-250

NAT1360 10G
EUR 103

10G Coomassie Blue G-250

NAT1362 10G
EUR 148

 

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