- Genetic symbol CRH
- Full name of the official gene Corticotropin-releasing hormone
- Alias symbols Corticoliberin, corticotropin-releasing factor, corticotropin-releasing factor, corticotropin-releasing factor, corticotropin-releasing hormone, CRF, Gm1347, LOC383938, MGC151298
- NCBI gene Id 12918
- protein name Corticoliberin
Description of Target
This gene encodes a member of the corticotropin-releasing factor family and a preproprotein that is proteolytically processed to generate a mature protein product.
This protein product is a neuropeptide hormone that binds to corticotropin-releasing hormone receptors (CRHR1 and CRHR2) to stimulate the release of adrenocorticotropic hormone from the pituitary gland in response to stress.
The encoded protein can also regulate angiogenesis and inflammation. Knockout mice homozygous for this gene exhibit reduced levels of corticosterone while the offspring of these mice die perinatally.
ELISA Kit Principle
The Aviva Systems Biology Crh ELISA Kit (Mouse) (OKEH00624) is based on a competitive enzyme immunoassay technique. The microwell plate of this kit has been pre-coated with an anti-dog Crh antibody. Samples or standards are added to the wells along with a fixed amount of biotinylated Crh and incubated.
The Crh found in the sample or standards compete with the biotinylated Crh for limited binding sites on the immobilized anti-dog Crh antibody. Excess unbound biotinylated Crh and the sample or standard Crh are washed from the plate. Add the avidin-HRP conjugate, incubate and wash. An enzymatic reaction then occurs by adding TMB substrate which is catalyzed by the immobilized HRP to generate a blue product that changes to yellow after adding an acid stop solution.
The density of the yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Crh captured in the well and inversely proportional to the amount of Crh that was contained in the sample or standard.