Cy5 Boc-hydrazide
Cy5 Boc-hydrazide is a boc-protected reactive dye containing a Boc-protected hydrazide. Boc protective group can be removed with TFA, or other strong acid to form the reactive hydrazide, which can then couple with various carbonyl groups yielding Cyanine5-labeled molecules.
| Boc-Dab(Boc)-OH·DCHA | |||
| 5-04713 | CHI Scientific | 1g | Ask for price |
| Boc-Dab(Boc)-OH·DCHA | |||
| 5-04714 | CHI Scientific | 5g | Ask for price |
| Boc-Dap(Boc)-OH·DCHA | |||
| 5-04787 | CHI Scientific | 5g | Ask for price |
| Boc-Dap(Boc)-OH·DCHA | |||
| 5-04788 | CHI Scientific | 25g | Ask for price |
| Boc-His(Boc)-OH | |||
| 5-05809 | CHI Scientific | 25g | Ask for price |
| Boc-His(Boc)-OH | |||
| 5-05810 | CHI Scientific | 100g | Ask for price |
| Boc-His(Boc)-OMe | |||
| 5-05813 | CHI Scientific | 25g | Ask for price |
| Boc-His(Boc)-OMe | |||
| 5-05814 | CHI Scientific | 100g | Ask for price |
| Boc-Lys(Boc)-OH | |||
| 5-05889 | CHI Scientific | 25g | Ask for price |
| Boc-Lys(Boc)-OH | |||
| 5-05890 | CHI Scientific | 100g | Ask for price |
| Boc-Lys(Boc)-OH·DCHA | |||
| 5-05893 | CHI Scientific | 25g | Ask for price |
| Boc-Lys(Boc)-OH·DCHA | |||
| 5-05894 | CHI Scientific | 100g | Ask for price |
| Boc-Lys(Boc)-OMe | |||
| 5-05895 | CHI Scientific | 25g | Ask for price |
| Boc-Lys(Boc)-OMe | |||
| 5-05896 | CHI Scientific | 100g | Ask for price |
| Boc-Lys(Boc)-ONp | |||
| 5-05897 | CHI Scientific | 5g | Ask for price |
| Boc-Lys(Boc)-ONp | |||
| 5-05898 | CHI Scientific | 25g | Ask for price |
| Boc-Lys(Boc)-OSu | |||
| 5-05899 | CHI Scientific | 5g | Ask for price |
| Boc-Lys(Boc)-OSu | |||
| 5-05900 | CHI Scientific | 25g | Ask for price |
| Boc-Trp(Boc)-OH | |||
| 5-06129 | CHI Scientific | 25g | Ask for price |
| Boc-Trp(Boc)-OH | |||
| 5-06130 | CHI Scientific | 100g | Ask for price |
| Boc-Dap(Boc)-OH.DCHA | |||
| A6722-100000 | ApexBio | 100 g | 1452 EUR |
| Boc-Dap(Boc)-OH.DCHA | |||
| A6722-25000 | ApexBio | 25 g | 756 EUR |
| Boc-Lys(Boc)-OSu | |||
| A-1025.0005 | Bachem | 5.0g | 211.2 EUR |
| Boc-Lys(Boc)-OSu | |||
| A-1025.0025 | Bachem | 25.0g | 733.2 EUR |
| Boc-Lys(Boc)-ONp | |||
| A-1030.0005 | Bachem | 5.0g | 138 EUR |
| Boc-Lys(Boc)-ONp | |||
| A-1030.0025 | Bachem | 25.0g | 441.6 EUR |
| Boc-Tyr(Boc)-OH | |||
| A-1040.0005 | Bachem | 5.0g | 166.8 EUR |
| Boc-Tyr(Boc)-OH | |||
| A-1040.0025 | Bachem | 25.0g | 572.4 EUR |
| Boc-Orn(Boc)-OH | |||
| A-2645.0005 | Bachem | 5.0g | 211.2 EUR |
| Boc-Orn(Boc)-OH | |||
| A-2645.0025 | Bachem | 25.0g | 704.4 EUR |
| Boc-Trp(Boc)-OH | |||
| A-4550.0005 | Bachem | 5.0g | 254.4 EUR |
| Boc-Trp(Boc)-OH | |||
| A-4550.0025 | Bachem | 25.0g | 907.2 EUR |
| Boc-Lys(Boc)-OSu | |||
| GM6002-25G | Glentham Life Sciences | 25 g | 332.4 EUR |
| BOC | |||
| GT15229 | Neuromics | 100 ug | 631.2 EUR |
| Fmoc-Lys(Boc)-Leu-Lys(Boc) [Fmoc-Lys(Boc)-Leu-Lys(Boc)] | |||
| SP-53660-1 | Alpha Diagnostics | 1 g | 1666.8 EUR |
| Boc-Arg(Boc)2-OH | |||
| 5-05563 | CHI Scientific | 5g | Ask for price |
| Boc-Arg(Boc)2-OH | |||
| 5-05564 | CHI Scientific | 25g | Ask for price |
| Boc-Lys(Boc)-Pro-OH | |||
| 5-05891 | CHI Scientific | 5g | Ask for price |
| Boc-Lys(Boc)-Pro-OH | |||
| 5-05892 | CHI Scientific | 25g | Ask for price |
| Boc-D-Lys(Boc)-OH | |||
| 5-05925 | CHI Scientific | 25g | Ask for price |
| Boc-D-Lys(Boc)-OH | |||
| 5-05926 | CHI Scientific | 100g | Ask for price |
| Boc-D-Lys(Boc)-OH·DCHA | |||
| 5-05927 | CHI Scientific | 25g | Ask for price |
| Boc-D-Lys(Boc)-OH·DCHA | |||
| 5-05928 | CHI Scientific | 100g | Ask for price |
| Boc-D-Lys(Boc)-ONp | |||
| 5-05929 | CHI Scientific | 25g | Ask for price |
| Boc-D-Lys(Boc)-ONp | |||
| 5-05930 | CHI Scientific | 100g | Ask for price |
| Boc-D-Lys(Boc)-OSu | |||
| 5-05931 | CHI Scientific | 5g | Ask for price |
| Boc-D-Lys(Boc)-OSu | |||
| 5-05932 | CHI Scientific | 25g | Ask for price |
| Boc-D-Trp(Boc)-OH | |||
| 5-06143 | CHI Scientific | 5g | Ask for price |
| Boc-D-Trp(Boc)-OH | |||
| 5-06144 | CHI Scientific | 25g | Ask for price |
| Boc-NH-PEG-NH-Boc | |||
| abx085208-10kDa1g | Abbexa | 10 kDa; 1 g | 760.8 EUR |
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Catalog: BP-22573
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Name: Cy5 Boc-hydrazide
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Formula: C37H49ClN4O3
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MW: 633.3
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CAS:
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Purity 96%
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Ships Within: 24 Hours
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Storage Condition: -20°C
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Solubility: DMSO, DMF, DCM
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Shipping: Ambient temperature
DESCRIPTION
Application
18:1 Cy5 PE or 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(Cyanine 5), chloroform has been used:
- as a standard for the estimation of absolute density of Cy5 labeled Trx-His6-NCav-CT on supported lipid bilayers (SLB)
- in annexin-coated vesicle production
- to label vesicle types in order to optically distinguish them from other vesicles and to initiate biochemical reactions in a cell-mimetic compartment
Biochem/physiol Actions
Packaging
SAFETY INFORMATION
Pictograms
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Inhalation – Acute Tox. 4 Oral – Aquatic Chronic 3 – Carc. 2 – Eye Irrit. 2 – Repr. 2 – Skin Irrit. 2 – STOT RE 1 – STOT SE 3
Target Organs
Central nervous system
Storage Class Code
6.1D – Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects.
WGK
WGK 3
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Cy5 dye (also sold under Sulfo-Cyanine5 name) is one of the most popular far-red fluorescent dyes. It is often a reagent of choice for protein and peptide labeling. Cy5 dye is a water-soluble, bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. Cy5 conjugates of antibodies, peptides, and proteins are pH insensitive from pH 4 to pH 10. A significant advantage to using long wavelength dyes such as Cy5 or AF 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.
The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. We do not recommend using Cy5 NHS ester for labeling proteins at high molar ration due significant self-quenching. Cy5 conjugates are recommended for detection of moderate-to-high abundance targets. For detection of low-abundance biological targets we recommend using APDye 647 (Alexa Fluor® 647 analog), or IR 650 dye (IRDye 650 analog), which can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.
AxisPharm offers 3000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
Grant information
We thank Dr Debby Feytens for some of the Cy5 precursor synthesis work and Dr Han-Shen Tae for the Cy5 control experiments. MM was supported by the European Research Council under the European Union’s Horizon 2020 research and innovation program (714366), by the Australian Research Council (DE150100784, DP190101667), and by the Vienna Science and Technology Fund (WWTF; LS18–053). PFA, RJL, and DJA were supported by Program Grant APP1072113 from the National Health & Medical Research Council (NHMRC) of Australia and NHMRC Principal Research Fellowships to PFA (APP1080593) and to RJL (APP1072113). Finally, we want to recognise Professor Paul Alewood’s outstanding contributions to the field of peptide science and thank him for his long-lasting collegiality, mentorship, and friendship.
- Synonyms：
Cy5.5-PEG-NHS
Cyanur5.5-PEG-Succinimidyl ester - Purity：
≥95% - Recommended Storage Condition：
Store at -5°C,keep in dry and avoid sunlight. - Uses：
Applicated in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. Monoclonal Anti-Rat IgG1-Cy5 Conjugate 50126-Cy5 Alpha Diagnostics 0.1 ml 489.6 EUR Hamster IgG-Cy5 conjugate, isotype control (Syrian) 20003-1-Cy5 Alpha Diagnostics 50 tests 270 EUR Goat IgG F(ab')2 fragment-Cy5 conjugate 20011-FAB2-Cy5 Alpha Diagnostics 50 tests 242.4 EUR Cy5 tertrazine [Cy5 tertrazine] 911 AAT Bioquest 1 mg 262.8 EUR CY5 HY-D0821 MedChemExpress 25mg 1795.2 EUR PE-Cy5 (Phycoerhthrin-Cy5 Conjugate) 7850-250 Biovision each 444 EUR Cy5 azide A8128-25 ApexBio 25 mg 2078.4 EUR Cy5 azide A8128-5 ApexBio 5 mg 741.6 EUR Cy5 phosphoramidite 6071 AAT Bioquest 100 umoles 60 EUR DBCO-Cy5 923 AAT Bioquest 1 mg 158.4 EUR CY5-SE HY-D0819 MedChemExpress 25mg 555.6 EUR CY5-YNE HY-D0820 MedChemExpress 10mM/1mL 943.2 EUR CY5-N3 HY-D0832 MedChemExpress 5mg 639.6 EUR Cy5-Dctp PA55021 Scientific Laboratory Supplies EACH 388.74 EUR Cy5-Dutp PA55022 Scientific Laboratory Supplies EACH 388.74 EUR Cy5-Ctp 25801087 Scientific Laboratory Supplies EACH 432.06 EUR Cy5 tyramide 11066 AAT Bioquest 1 mg 109 EUR Cy5 aldehyde 9011 AAT Bioquest 5 mg 318 EUR Cy5 aldehyde 9012 AAT Bioquest 1 mg 203 EUR Cy5 tetrazine 902 AAT Bioquest 1 mg 222 EUR Cy5 NHS ester A8108-25 ApexBio 25 mg 895.2 EUR Cy5 NHS ester A8108-5 ApexBio 5 mg 351.6 EUR Cy5 carboxylic acid A8137-25 ApexBio 25 mg 1243.2 EUR Cy5 carboxylic acid A8137-5 ApexBio 5 mg 630 EUR CD117 antibody (CY5) 61R-CD117bMSCY5 Fitzgerald 100 ug 495.6 EUR CD11b antibody (CY5) 61R-CD11baMSCY5 Fitzgerald 100 ug 495.6 EUR CD19 antibody (CY5) 61R-CD19aMSCY5 Fitzgerald 100 ug 522 EUR CD19 antibody (CY5) 61R-CD19cHUCY5 Fitzgerald 100 tests 522 EUR CD3e antibody (CY5) 61R-CD3egMSCY Fitzgerald 100 ug 340.8 EUR CD3e antibody (CY5) 61R-CD3ehMSCY Fitzgerald 100 ug 340.8 EUR CD3 antibody (CY5) 61R-CD3gHUCY5 Fitzgerald 100 tests 535.2 EUR CD45.1 antibody (CY5) 61R-CD45gMSCY5 Fitzgerald 100 ug 548.4 EUR CD45.2 antibody (CY5) 61R-CD45hMSCY5 Fitzgerald 100 ug 457.2 EUR CD45R antibody (CY5) 61R-CD45RcMSCY5 Fitzgerald 100 ug 495.6 EUR CD4 antibody (CY5) 61R-CD4eMSCY Fitzgerald 100 ug 483.6 EUR CD4 antibody (CY5) 61R-CD4kHUCY5 Fitzgerald 100 tests 522 EUR CD8a antibody (CY5) 61R-CD8abMSCY5 Fitzgerald 100 ug 495.6 EUR CD8a antibody (CY5) 61R-CD8adCKCY5 Fitzgerald 100 ug 651.6 EUR CD8a antibody (CY5) 61R-CD8aeCKCY5 Fitzgerald 100 ug 548.4 EUR Azido-Cy5 tyramide 11061 AAT Bioquest 1 mg 367.2 EUR PE-Cy5 Tandem 2610 AAT Bioquest 1 mg 262.8 EUR Cy5-PEG-SH abx085228-1kDa50mg Abbexa 1 kDa; 50 mg 1045.2 EUR Cy5-PEG-SH abx085228-2kDa50mg Abbexa 2 kDa; 50 mg 1095.6 EUR Cy5-PEG-SH abx085228-34kDa50mg Abbexa 3.4 kDa; 50 mg 1128 EUR Cy5-PEG-SH abx085228-5kDa50mg Abbexa 5 kDa; 50 mg 1128 EUR Cy5-PEG-COOH abx085357-10kDa50mg Abbexa 10 kDa; 50 mg 598.8 EUR Cy5-PEG-COOH abx085357-20kDa50mg Abbexa 20 kDa; 50 mg 598.8 EUR Cy5-PEG-COOH abx085357-2kDa50mg Abbexa 2 kDa; 50 mg 642 EUR Cy5-PEG-COOH abx085357-5kDa50mg Abbexa 5 kDa; 50 mg 642 EUR Cy5-PEG-Mal abx085358-10kDa50mg Abbexa 10 kDa; 50 mg 655.2 EUR
Cyanine5 Boc-hydrazide, 1 mg
| Hersteller | Lumiprobe |
|---|---|
| Kategorie | |
| Typ | Reagents |
| Specific against | other |
| Menge | 1mg |
| ArtNr | LPB-130A0 |
| eClass 6.1 | 32160401 |
| eClass 9.0 | 42051003 |
In-solution conjugation is the most commonly used strategy to label peptides and proteins with fluorophores. However, lack of site-specific control and high costs of fluorophores are recognised limitations of this approach. Here, we established facile access to grams of Cy5-COOH via a two-step synthetic route, demonstrated that Cy5 is stable to HF treatment and therefore compatible with Boc-SPPS, and coupled Cy5 to the N-terminus of α-conotoxin RgIA while still attached to the resin. Folding of the two-disulfide containing Cy5-RgIA benefitted from the hydrophobic nature of Cy5 resulting in only the globular disulfide bond isomer. In contrast, wild-type α-RgIA folded into the inactive ribbon and bioactive globular isomer under the same conditions. Labelled α-RgIA retained its ability to inhibit acetylcholine(100 μM)-evoked current reversibly with an IC50 of 5.0 nM (Hill coefficient = 1.7) for α-RgIA and an IC50 of 1.6 (Hill coefficient = 1.2) for Cy5-RgIA at the α9α10 nicotinic acetylcholine receptors (nAChRs) heterologeously expressed in Xenopus oocytes. Cy5-RgIA was then used to successfully visualise nAChRs in RAW264.7 mouse macrophage cell line. This work introduced not only a new and valuable nAChR probe, but also a new versatile synthetic strategy that facilitates production of milligram to gram quantities of fluorophore-labelled peptides at low cost, which is often required for in vivo experiments. The strategy is compatible with Boc- and Fmoc-chemistry, allows for site-specific labelling of free amines anywhere in the peptide sequence, and can also be used for the introduction of Cy3/Cy5 FRET pairs.
