In diabetes-induced intervertebral disc degeneration (Db-IVDD), senescence and apoptosis of nucleus pulposus cells (NPCs) are main contributing components. Telomere attrition and telomerase downregulation are a number of the foremost causes for senescence and eventual apoptosis.
The derivatives of the Chinese language herb Astragalus membranaceus, Cycloastragenol (CAG) and Astragaloside IV (AG-IV), are reportedly efficient telomerase activators towards telomere shortening; nevertheless, their impact in Db-IVDD haven’t been explored.
The current examine concurrently investigated the regulation of those derivatives on senescence, apoptosis, telomeres and telomerase a mannequin of high-glucose (HG)-induced stress utilizing rat main NPCs.
The NPCs had been stimulated with HG (50 mM) to evoke HG-induced stress, and the results of CAG and AG-IV had been noticed on:
i) The expression degree of senescence marker p16;
ii) β-Gal staining;
iii) the expression ranges of apoptosis markers cleaved-caspase 3 (c-C3), BAX and Bcl-2; iv) telomerase activation with telomerase reverse transcriptase (TERT) mRNA and protein expression, whereas telomere size was measured with reverse transcription-quantitative PCR.
Cell proliferation was decided utilizing the Cell Counting Equipment-Eight assay. Outcomes demonstrated an upregulation within the expression ranges of p16, c-C3 and BAX, and elevated β-Gal staining; whereas the expression degree of Bcl-2 was downregulated in a concentration-dependent method.
Pre-treatment of the NPCs with CAG and AG-IV downregulated the protein expression ranges of p16, c-C3 and BAX, and decreased the share of β-Gal and FITC staining; whereas upregulating the Bcl-2 expression. These results protected the cells from HG stress-induced senescence and apoptosis.
HG additionally downregulated the expression profile of TERT and shortened the telomere size in a glucose concentration-dependent method. Whereas pretreatment with CAG and AG-IV upregulated TERT expression and ameliorated the telomere attrition. CAG and AG-IV additionally elevated cell proliferation and improved cell morphology in HG circumstances.
General, these findings indicated that CAG and AG-IV suppressed HG stress-induced senescence and apoptosis, along with enhancing telomerase activation and lengthening of the Telomere.
Due to this fact, CAG and AG-IV extended the replicative functionality and longevity of the NPCs and so they have the potential to be therapeutic brokers in Db-IVDD.
Repetitive spikes of glucose and lipid induce senescence-like phenotypes of bone marrow stem cells via H3K27me3 demethylase-mediated epigenetic regulation
Bone marrow-derived endothelial progenitor cells (EPCs) contribute to endothelial restore and angiogenesis. Decreased variety of circulating EPCs is related to future cardiovascular occasions. We examined whether or not dysregulated glucose and/or triglyceride (TG) metabolism has an impression on EPC homeostasis.
The evaluation of metabolic components related to circulating EPC quantity in people revealed that postprandial hyperglycemia is negatively correlated with circulating EPC quantity and this correlation seems to be additional enhanced within the presence of postprandial hypertriglyceridemia (hTG).
We due to this fact examined the impact of glucose/TG spikes on bone marrow lineage-sca-1+c-equipment+ (LSK) cells in mice, as a result of primitive EPCs reside in bone marrow LSK fraction.Repetitive glucose+lipid (GL) spikes, however not glucose (G) or lipid (L) spikes alone, induced senescence-like phenotypes of LSK cells, and this phenomenon was reversible after cessation of GL spikes.
G spikes and GL spikes differentially affected transcriptional program of LSK cell metabolism and differentiation. GL spikes upregulated a histone H3K27 demethylase JMJD3, and inhibition of JMJD3 eradicated GL spikes-induced LSK cell senescence-like phenotypes.
These observations counsel that postprandial glucose/TG dysmetabolism modulate transcriptional regulation in LSK cells via H3K27 demethylase-mediated epigenetic regulation, resulting in senescence-like phenotypes of LSK cells, decreased variety of circulating EPCs, and growth of atherosclerotic heart problems.
miR-1260b, mediated by YY1, prompts KIT signaling by concentrating on SOCS6 to manage cell proliferation and apoptosis in NSCLC.
Non-small cell lung most cancers (NSCLC) is likely one of the most typical aggressive malignancies. miRNAs have been recognized as necessary biomarkers and regulators of NSCLC. Nevertheless, the purposeful contributions of miR-1260b to NSCLC cell proliferation and apoptosis haven’t been studied.
On this examine, miR-1260b was upregulated in NSCLC plasma, tissues, and cell strains, and its excessive expression was correlated with tumor dimension and development. Functionally, miR-1260b overexpression promoted cell proliferation and cell cycle, conversely inhibited cell apoptosis and senescence.
Mechanically, miR-1260b negatively regulated SOCS6 by straight binding to its 3′-UTR. Moreover, miR-1260b-mediated suppression of SOCS6 activated KIT signaling. Furthermore, YY1 was an upstream regulator of miR-1260b.
This examine is the primary as an example that miR-1260b, mediated by YY1, prompts KIT signaling by concentrating on SOCS6 to manage NSCLC cell proliferation and apoptosis, and is a possible biomarker and therapeutic goal for NSCLC.
In sum, our work offers new insights into the molecular mechanisms of NSCLC concerned in cell proliferation and apoptosis.
Lumican silencing alleviates tumor necrosis factor-α-induced nucleus pulposus cell irritation and senescence by inhibiting apoptosis sign regulating kinase 1/p38 signaling pathway through inactivating Fas ligand expression
A latest examine has reported that lumican (LUM) is expressed at a excessive degree within the nucleus pulposus specimens from herniated lumbar disc, with out description of the precise mechanism.
This examine was designed to analyze the operate and mechanism of LUM in intervertebral disc degeneration (IDD). On this examine, human nucleus pulposus cells (hNPCs) cells had been challenged with tumor necrosis issue (TNF)-α to ascertain the IDD in vitro mannequin.
After LUM silencing, cell viability was detected utilizing CCK-8 equipment, and the expression of inflammatory components was evaluated utilizing RT-qPCR and ELISA. Move cytometry and β-galactosidase staining had been used to find out cell cycle and cell senescence.
The expression of cycle and senescence-related proteins was evaluated with western blotting. Then, Fas ligand (FasL) was overexpressed and proteins in apoptosis sign regulating kinase 1 (ASK1)/p38 signaling had been examined. Lastly, GS-4997, an inhibitor of ASK1, was used to discover the regulatory results of LUM on ASK1/p38 signaling in TNF-α-induced hNPCs.
Outcomes indicated that LUM expression was upregulated in TNF-α-challenged hNPCs. LUM gene interference mitigated TNF-α-induced inflammatory response, cell cycle arrest, and senescence of hNPCs.
It was then discovered that LUM silencing may inhibit ASK1/p38 signaling in TNF-α-treated hNPCs, which was reversed by FasL overexpression. Moreover, ASK1/p38 participated within the mediation by LUM of TNF-α-induced irritation, cell cycle arrest, and senescence of hNPCs.
Regucalcin (Senescence Marker Protein-30) Antibody |
|||
| 20-abx115098 | Abbexa |
|
|
96-well Cellular Senescence Assay (SA ?-Gal Activity) |
|||
| CBA-231 | Cell Biolabs | 120 assays | EUR 762 |
|
Description: Our Cellular Senescence Activity Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Quantify senescence using a fluorescence plate reader. |
|||
96-well Cellular Senescence Assay (SA ?-Gal Activity) |
|||
| CBA-231-5 | Cell Biolabs | 5 x 120 assays | EUR 3177.6 |
|
Description: Our Cellular Senescence Activity Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Quantify senescence using a fluorescence plate reader. |
|||
Cell Meterâ„¢ Cellular Senescence Activity Assay Kit *Red Fluorescence* |
|||
| 23007-100Tests | AAT Bioquest | 100 Tests | EUR 372 |
|
Description: Cellular Senescence is an irreversible growth arrest triggered in order to prevent growth in DNA damaged cells. |
|||
Cell Meterâ„¢ Cellular Senescence Activity Assay Kit *Green Fluorescence* |
|||
| 23005-100Tests | AAT Bioquest | 100 Tests | EUR 334 |
|
Description: Cellular Senescence is an irreversible growth arrest triggered in order to prevent growth in DNA damaged cells. |
|||
Cell Meter™ Cellular Senescence Activity Assay Kit *Red Fluorescence* |
|||
| 23007 | AAT Bioquest | 100 Tests | EUR 372 |
96-Well Cellular Senescence Assay Kit (SA-?-gal Activity, Fluorometric Format), Trial Size |
|||
| CBA-231-T | Cell Biolabs | 24 assays | EUR 414 |
|
Description: Our Cellular Senescence Activity Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Quantify senescence using a fluorescence plate reader. |
|||
Human LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) CLIA Kit |
|||
| 20-abx496142 | Abbexa |
|
|
Human LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) ELISA Kit |
|||
| 20-abx258138 | Abbexa |
|
|
Human LIM And Senescent Cell Antigen Like Domains Protein 1 ELISA Kit (LIMS1) |
|||
| RK01780 | Abclonal | 96 Tests | EUR 625.2 |
Human LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) ELISA Kit |
|||
| SEP882Hu-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 6227.58 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) in tissue homogenates, cell lysates and other biological fluids. |
|||
Human LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) ELISA Kit |
|||
| SEP882Hu-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 618.04 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) in tissue homogenates, cell lysates and other biological fluids. |
|||
Human LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) ELISA Kit |
|||
| SEP882Hu-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 831.48 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) in tissue homogenates, cell lysates and other biological fluids. |
|||
Human LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) ELISA Kit |
|||
| SEP882Hu-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 3381.66 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) in tissue homogenates, cell lysates and other biological fluids. |
|||
Human LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) ELISA Kit |
|||
| 4-SEP882Hu | Cloud-Clone |
|
|
|
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. |
|||
ELISA kit for Human LIMS1 (LIM And Senescent Cell Antigen Like Domains Protein 1) |
|||
| ELK7399 | ELK Biotech | 1 plate of 96 wells | EUR 518.4 |
|
Description: A sandwich ELISA kit for detection of LIM And Senescent Cell Antigen Like Domains Protein 1 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
|||
LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) Antibody |
|||
| 20-abx211766 | Abbexa |
|
|
LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) Antibody |
|||
| 20-abx213338 | Abbexa |
|
|
LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) Antibody |
|||
| 20-abx113502 | Abbexa |
|
|
LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) Antibody |
|||
| 20-abx173352 | Abbexa |
|
|
LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) Antibody |
|||
| 20-abx104616 | Abbexa |
|
|
LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) Antibody |
|||
| 20-abx338852 | Abbexa |
|
|
ELISA kit for Mouse LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1) |
|||
| KTE71175-48T | Abbkine | 48T | EUR 398.4 |
|
Description: Quantitative sandwich ELISA for measuring Mouse LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
|||
ELISA kit for Mouse LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1) |
|||
| KTE71175-5platesof96wells | Abbkine | 5 plates of 96 wells | EUR 2538 |
|
Description: Quantitative sandwich ELISA for measuring Mouse LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
|||
ELISA kit for Mouse LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1) |
|||
| KTE71175-96T | Abbkine | 96T | EUR 646.8 |
|
Description: Quantitative sandwich ELISA for measuring Mouse LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
|||
LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) Antibody (HRP) |
|||
| 20-abx336372 | Abbexa |
|
|
Human LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) Protein |
|||
| 20-abx067782 | Abbexa |
|
|
LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) Antibody (FITC) |
|||
| 20-abx336373 | Abbexa |
|
|
LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) Antibody (Biotin) |
|||
| 20-abx336374 | Abbexa |
|
|
Recombinant LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) |
|||
| 4-RPP882Hu01 | Cloud-Clone |
|
|
|
Description: Recombinant Human LIM And Senescent Cell Antigen Like Domains Protein 1 expressed in: E.coli |
|||
Mouse LIM and senescent cell antigen-like-containing domain protein 1 (Lims1) |
|||
| 1-CSB-BP859129MO | Cusabio |
|
|
|
Description: Recombinant Mouse LIM and senescent cell antigen-like-containing domain protein 1(Lims1) expressed in Baculovirus |
|||
Human LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1) |
|||
| 1-CSB-EP232922HU | Cusabio |
|
|
|
Description: Recombinant Human LIM and senescent cell antigen-like-containing domain protein 1(LIMS1) expressed in E.coli |
|||
Mouse LIM and senescent cell antigen-like-containing domain protein 1 (Lims1) |
|||
| 1-CSB-EP859129MO | Cusabio |
|
|
|
Description: Recombinant Mouse LIM and senescent cell antigen-like-containing domain protein 1(Lims1) expressed in E.coli |
|||
LIM And Senescent Cell Antigen-Like-Containing Domain Protein 3 (LIMS3) Antibody |
|||
| abx037172-100ug | Abbexa | 100 ug | EUR 469.2 |
LIM And Senescent Cell Antigen-Like-Containing Domain Protein 3 (LIMS3) Antibody |
|||
| 20-abx304358 | Abbexa |
|
|
LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) Polyclonal Antibody (Human, Mouse, Pig) |
|||
| 4-PAP882Hu01 | Cloud-Clone |
|
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse, Pig LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) |
|||
LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) Polyclonal Antibody (Human, Mouse, Pig), APC |
|||
| 4-PAP882Hu01-APC | Cloud-Clone |
|
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse, Pig LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1). This antibody is labeled with APC. |
|||
LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) Polyclonal Antibody (Human, Mouse, Pig), Cy3 |
|||
| 4-PAP882Hu01-Cy3 | Cloud-Clone |
|
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse, Pig LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1). This antibody is labeled with Cy3. |
|||
LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) Polyclonal Antibody (Human, Mouse, Pig), HRP |
|||
| 4-PAP882Hu01-HRP | Cloud-Clone |
|
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse, Pig LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1). This antibody is labeled with HRP. |
|||
LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) Polyclonal Antibody (Human, Mouse, Pig), PE |
|||
| 4-PAP882Hu01-PE | Cloud-Clone |
|
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse, Pig LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1). This antibody is labeled with PE. |
|||
LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) Polyclonal Antibody (Human, Mouse, Pig), FITC |
|||
| 4-PAP882Hu01-FITC | Cloud-Clone |
|
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse, Pig LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1). This antibody is labeled with FITC. |
|||
LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) Polyclonal Antibody (Human, Mouse, Pig), APC-Cy7 |
|||
| 4-PAP882Hu01-APC-Cy7 | Cloud-Clone |
|
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse, Pig LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1). This antibody is labeled with APC-Cy7. |
|||
LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) Polyclonal Antibody (Human, Mouse, Pig), Biotinylated |
|||
| 4-PAP882Hu01-Biotin | Cloud-Clone |
|
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse, Pig LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1). This antibody is labeled with Biotin. |
|||
To conclude, interference with LUM successfully mitigated TNF-α-induced inflammatory response, cell cycle arrest, and cell senescence. Additional experiments confirmed the involvement of ASK1/p38 pathway in LUM-mediated NP cell phenotypes via FasL.
