This kit is a solid-phase sandwich enzyme-linked immunosorbent assay (ELISA). Samples, including standards for known and unknown target protein concentrations, are pipetted into these wells. During the first incubation, the target protein antigen and a biotinylated monoclonal antibody specific for the target protein are incubated simultaneously. After washing, the enzyme (streptavidin-peroxidase) is added. After incubation and washing to remove all unbound enzymes, a substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the target protein concentration present in the samples.
Antigen name: Osteopontin OPN
Detection range: 0.156 ng / ml-10 ng / ml
Sensitivity: 0.04 ng / ml
Unit Size: 96T
Safety statements: for research use only.
Sample availability: No
Information selected from the online data sheet is extracted from bioinformatics databases, occasionally resulting in ambiguous or irrelevant product information. It is the customer’s responsibility to review, verify and evaluate the information to make sure it matches their requirements before purchasing the kit. The essays in our ELISA kit are dynamic research tools and can sometimes be updated and improved.
If the format of this assay is important to you, please request the current manual or contact our technical support team with a pre-sale consultation before placing an order. We will confirm the current details of the trial. We cannot guarantee that the sample manual posted online is the most current manual, it is intended to serve as an example only. See the instructions for use provided with the test kit for precise details.
Occasionally, small volumes of OPN ELISA kit vials may get caught in the product vial seal during shipping and storage. If necessary, briefly centrifuge the vial in a benchtop centrifuge to dislodge any liquid on the lid of the container. Some products may need to be shipped with dry ice and an additional fee may apply for dry ice.
Search terms for OPN purchase
MBS1604306 is a ready-to-use microwell plate ELISA (enzyme-linked immunosorbent assay) kit for testing the presence of target analytes from the Osteopontin, OPN, ELISA kit in biological samples. The concentration gradients of the kit standards or positive controls provide a theoretical detection range of the kit in biological research samples containing OPN.
The analytical biochemical ELISA technique of the MBS1604306 kit is based on OPN antibody-OPN antigen interactions (immunoadsorption) and an HRP colorimetric detection system to detect OPN antigen targets in samples. The ELISA Kit is designed to detect native, non-recombinant OPN. Appropriate sample types may include secretions and undiluted body fluids and/or tissue homogenates. Quality control trials evaluating reproducibility identified the intra-assay CV (%) and inter-assay CV (%).