Quality level: 200
Use: mL enough for 100 mini-gels
Concentration: 10,000 × in DMSO
Storage temperature: −20 ° C
SYBR Green I is an asymmetric cyanine stain, which is used to detect nucleic acids. It consists of an N-alkylated benzothiazole or benzoxazole ring system, which is linked by a monomethine bridge to a pyridinium or quinolinium ring system. SYBR Green I binds to the minor groove of the dsDNA and is excited at a wavelength of 480 nm. It has a fluorescence emission peak of 520 nm.
SYBR Green I nucleic acid gel staining has been used for real-time PCR quantification and flow cytometry. SYBR Green I Nucleic Acid Gel Stain has been used to:
- dsDNA quantification
- to stain DNA in the polymerase chain reaction (PCR)
- for kite test technique
- to assess the integrity of the sperm membrane
A proprietary asymmetric cyanine stain that is an ultrasensitive stain for post-electrophoresis staining of dsDNA in agarose or polyacrylamide gels. SYBR Green I can also be used to detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any pre-wash step, although the sensitivity is lower (approximately 100 to 300 pg per band using 254 nm epi-illumination). SYBR Green II is recommended for ss-DNA and RNA.
SYBR Green I stain has been shown to be much less mutagenic than ethidium bromide in Ames tests. SYBR Green I is also a very sensitive stain for oligonucleotides, allowing the detection of as little as 1-2 ng of a synthetic 24-mer on a 5% polyacrylamide gel, which has a sensitivity 50-100 times greater than that obtained with ethidium bromide. SYBR Green I staining of agarose gels does not interfere with nucleic acid transfer to membranes or subsequent hybridization in Southern or Northern blot analysis as long as 0.1% -0.3% SDS is included in the prehybridization and hybridization buffers to remove the dye.
Sigma Life Science is committed to bringing you greener alternative products, which adhere to one or more of the 12 principles of greener chemistry. This product has inherently safer chemistry, compared to using standard ethidium bromide for dyeing. For more information, see the post found in the related content.
Storage and stability
Store the product at –20 ° C. The diluted staining solution can be stored, protected from light, at 2–8 ° C for several weeks or at room temperature for 3–4 days.
- US Patent No. 5,436,134. Licensed by Molecular Probes, Inc.
- SYBR is a registered trademark of Life Technologies