Major Change Notification Note: HEK 293 HCP ELISA Kit, 3G 1 Refill
The expression of viral vectors in HEK 293 cells is a widely used procedure to obtain sufficient quantities of the desired virus. The manufacturing and purification process of these products leaves the potential for host cell protein (HCP) impurities from HEK 293 cells. HCP impurities can result in toxic or immunological adverse reactions and are therefore desirable to reduce these impurities to the lowest practical levels.
The polyclonal antibodies used in this kit have been generated and affinity purified using a soft lysate of HEK 293 cells to obtain HCPs which are typically in the initial stages of product recovery. This kit can be used as a process development tool to monitor optimal removal of host cell impurities, as well as in routine end-product release testing when comprehensively characterized using samples from your process.
The new Resupply 1 antibodies have recently been generated for an ELISA format to match as closely as possible the current performance of the F650S kit. Like the anti-HEK 293 HCP antibodies used in the current F650S kit, the new antibodies have broad coverage of ~ 90% for HEK 293 HCP as determined by antibody affinity extraction with 2D PAGE / Silver Stain and LC-MS.
ELISA HEK 293 HCP Kit, 3G, Refill 1, Cat. No. F650S will replace the current ELISA HEK 293 HCP Kit, 3G, Cat. No F650S. Kits containing the new replenishment antibodies will maintain the same catalogue number, F650S, and will be identified by lot numbers beginning with the characters “R1”. Both kits follow the same assay protocol and have a LOQ of 1 ng / mL. Use sample diluent, cat. No. I700, with these kits.
Product Type: ELISA kit
Standards -10 ° C to -30 ° C upon receipt, All other reagents in the kit 2 ° C to 8 ° C
Target expression system: HEK 293
Species group: Mammals
- Stop solution
- TBS Wash Concentrate, 20X
- TMB substrate
- Anti-HEK 293: HRP Conjugate
- HEK 293 HCP (A-F) Standards
- Anti-HEK 293 Coated Microtiter Strips