Easy Staining Process: Histofine One-Step Polymer Detection System
Histofine Easy Stain MAX PO is a detection reagent designed particularly to permit immunohistochemical staining on formalin-fixed paraffin-embedded human tissue sections. It’s the labeled polymer ready by combining amino acid polymers with peroxidase (PO) and secondary antibody which is decreased to Fab’ fragment. To remove background staining, solid-phase absorption of secondary antibody is carried out with human serum.
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We outlined the staining applicability of Histofine MOUSESTAIN, Mouse MAX-PO and Rat MAX-PO for fastened frozen and recent frozen tissue sections moreover paraffin embedded tissue sections. Two various kinds of cool air dried tissue sections, recent frozen tissues and glued frozen tissues of nine-week outdated Slc: ICR mouse and nine-week outdated Slc: SD rat, had been ready for immunohistochemistry (IHC) staining in accordance with standard technique.
Further two steps of 1 and a couple of to our really useful process for paraffin embedded tissue sections had been examined as follows:
STEP 1: Alter response time of polymer; STEP 2: Add 0.2% of glutaraldehyde (GA) answer earlier than the STEP 1, when background staining remains to be noticed by STEP 1. The steps 1 and a couple of resulted in elimination of background staining of most Histofine collection. Subsequently, polymer detection techniques, Histofine collection, are relevant for IHC staining of two various kinds of frozen tissue sections in addition to paraffin embedded tissue sections.