Human Collagen alpha-1 (X)

Product name

collagen, type X, alpha 1 (COL10A1), ELISA kit

Full Product Name

Human Collagen Alpha-1 (X) Chain, ELISA Kit COL10A1

Product synonym names

Human collagen alpha-1 (X) chain ELISA kit (COL10A1); OTTHUMP00000017049; Schmid metaphyseal chondrodysplasia; collagen X; alpha-1 polypeptide; type X alpha 1 collagen; type X collagen; alpha 1

Product gene name

COL10A1 Elisa kit

OMIM: gen 156500

3D structure

ModBase 3D Structure for Q03692

Species reactivity: Human

Specificity

This assay has high sensitivity and excellent specificity for the detection of human COL10A1. No significant cross-reactivity or interference was observed between human COL10A1 and analogues.

Samples

Serum, plasma, tissue homogenates, cell lysates

Test type: Sandwich

Detection range

0.312 ng / ml-20 ng / ml

Sensitivity

0.078 ng / ml

Detection wavelength: 450 nm

Sample volume: 50-100ul

Preparation and storage

Unopened test kits should be stored at 2-8 degrees C upon receipt. See pdf manual for further storage instructions.

ISO certification

Manufactured in an ISO 9001: 2015 certified laboratory.

Product note

Information selected from the online data sheet is extracted from bioinformatics databases, occasionally resulting in the ambiguous or irrelevant product information. It is the customer’s responsibility to review, verify and evaluate the information to make sure it matches their requirements before purchasing the kit. The essays in our ELISA kit are dynamic research tools and can sometimes be updated and improved.

If the format of this assay is important to you, please request the current manual or contact our technical support team with a pre-sale consultation before placing an order. We will confirm the current details of the trial. We cannot guarantee that the sample manual posted online is the most current manual, it is intended to serve as an example only.

Other notes

Small volumes of COL10A1 Elisa kit vial (s) may occasionally get caught in the product vial seal during shipping and storage. If necessary, briefly centrifuge the vial in a benchtop centrifuge to dislodge any liquid on the lid of the container. Some products may need to be shipped with dry ice and an additional fee may apply for dry ice.

Search terms for purchasing COL10A1

MBS917063 is a ready-to-use microwell plate ELISA (enzyme-linked immunosorbent assay) kit for testing for the presence of target analytes of the collagen, type X, alpha 1 (COL10A1) ELISA kit in biological samples. The concentration gradients of the kit standards or positive controls give a theoretical detection range of the kit in biological research samples containing COL10A1.

The analytical biochemical ELISA technique of the MBS917063 kit is based on COL10A1 antibody-COL10A1 antigen interactions (immunosorbent) and an HRP colourimetric detection system to detect COL10A1 antigen targets in samples. The ELISA Kit is designed to detect native, non-recombinant COL10A1. Appropriate sample types may include secretions and undiluted body fluids and/or tissue homogenates. Quality control trials evaluating reproducibility identified the intra-assay CV (%) and inter-assay CV (%).

Related Product Information for COL10A1 kit Elisa

Assay Principle: This assay employs the quantitative sandwich enzyme immunoassay technique. A microplate has been pre-coated with an antibody specific for COL10A1. Standards and samples are pipetted into the wells and any COL10A1 present binds to the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for COL10A1 is added to the wells.

After washing, avidin-conjugated horseradish peroxidase (HRP) is added to the wells. After washing to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and the colour develops in proportion to the amount of COL10A1 bound in the initial step. Colour development is stopped and the intensity of the colour is measured.

Precautions

All MyBioSource products are for scientific laboratory research purposes and not for diagnostic, therapeutic, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Product purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order when we reasonably believe that the intended use will be outside of our acceptable guidelines.

Disclaimer

While every effort has been made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be responsible for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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