A chimeric vesicular stomatitis virus with the glycoprotein of the lymphocytic choriomeningitis virus, VSV-GP, is a potent viral vaccine vector that overcomes a number of of the constraints of wild-type VSV.
Right here, we evaluated the potential of VSV-GP as an HIV vaccine vector. We launched genes for various variants of the HIV-1 envelope protein Env, i.e., secreted or membrane-anchored, intact or mutated furin cleavage web site or completely different C-termini, into the genome of VSV-GP.
We discovered that the addition of the Env antigen didn’t attenuate VSV-GP replication. All HIV-1 Env variants have been expressed in VSV-GP contaminated cells and a few have been integrated very effectively into VSV-GP particles. Essential epitopes for binding of broadly neutralizing antibodies in opposition to HIV-1 comparable to MPER (membrane-proximal exterior area), CD4 binding web site, V1V2 and V3 loop have been current on the floor of VSV-GP-Env particles.
Binding of quaternary antibodies indicated a trimeric construction of VSV-GP integrated Env. We detected excessive HIV-1 antibody titers in mice and confirmed that vectors expressing membrane-anchored Env elicited greater antibody titers than vectors that secreted Envs.
In rabbits, Tier 1A HIV-1 neutralizing antibodies have been detectable after prime immunization and titers additional elevated after boosting with a second immunization. Taken collectively, VSV-GP-Env is a promising vector vaccine in opposition to HIV-1 an infection since this vector permits incorporation of native monomeric and/or trimeric HIV-1 Env right into a viral membrane.
The antibodies in opposition to 5-bromo-2′-deoxyuridine particularly acknowledge trifluridine integrated into DNA.
Trifluridine (FTD) is a key element of the novel oral antitumor drug TAS-102 (additionally named TFTD), which consists of FTD and a thymidine phosphorylase inhibitor. FTD is meant to exert its cytotoxicity by way of large misincorporation into DNA, however the underlying mechanism of FTD incorporation into DNA and its correlation with cytotoxicity should not absolutely understood.
The current research exhibits that a number of antibodies in opposition to 5-bromo-2′-deoxyuridine (BrdU) particularly cross-react with FTD, both anchored to bovine serum albumin or integrated into DNA.
These antibodies are helpful for a number of organic purposes, comparable to fluorescence-activated cell sorting, fluorescent immunostaining and immunogold detection for electron microscopy. These strategies confirmed that FTD is principally integrated within the nucleus throughout S part in a concentration-dependent method.
As well as, FTD was additionally detected by immunohistochemical staining in paraffin-embedded HCT-116 xenograft tumors after intraperitoneal administration of FTD. Intriguingly, FTD was hardly detected in surrounding matrices, which consisted of fibroblasts with marginal expression of the nucleoside transporter genes SLC29A1 and SLC29A2.
Thus, purposes utilizing anti-BrdU antibodies will present highly effective instruments to unveil the underlying mechanism of FTD motion and to foretell or consider the efficacy and antagonistic results of TAS-102 clinically.
Quantitation of HLA proteins integrated by human immunodeficiency virus kind 1 and evaluation of neutralizing exercise of anti-HLA antibodies.
Human anti-human leukocyte antigen (HLA) antibodies have been assessed for neutralizing exercise in opposition to human immunodeficiency virus kind 1 (HIV-1) carrying HLA alleles with matching specificity. Multiparous girls carrying anti-HLA antibodies have been recognized.
Plasma samples from these girls have been confirmed as having antibodies that particularly certain to HLA proteins expressed on the peripheral blood mononuclear cells (PBMCs) of their husbands. A major HIV-1 isolate was cultured within the husband’s PBMCs in order that the virus carried matching HLA alleles.
To find out the HIV-1-neutralizing exercise of anti-HLA antibodies, the infectivity of the virus for GHOST cells (which specific inexperienced fluorescent protein after HIV an infection) was investigated within the presence of a plasma pattern optimistic for the respective anti-HLA antibody.
A neutralization assay was additionally carried out utilizing purified immunoglobulin G (IgG) from two plasma samples, and two plasma samples have been investigated within the presence of complement. The prerequisite for anti-HLA antibody-mediated neutralization is incorporation of HLA proteins by HIV-1.
Subsequently, the extent of incorporation of HLA proteins by the first HIV-1 isolate was estimated. The ratios of HLA class I protein to HIV-1 capsid (p24) protein cultured within the PBMCs of two wholesome people have been 0.017 and 0.054.
These ratios advised that the HIV-1 pressure used within the assay integrated extra HLA proteins than gp160 trimers. Anti-HLA antibody-positive plasma was discovered to comprise antibodies that particularly reacted to HIV-1 carrying cognate HLA alleles.
Nevertheless, incubation of HIV-1 with anti-HLA antibody- optimistic plasma or purified IgG didn’t present a discount in viral infectivity. HIV-1-neutralizing exercise was additionally not detected within the presence of complement.
This research exhibits that HIV-1 major isolates cultured in PBMCs comprise important quantities of HLA proteins. Nevertheless, the binding of antibodies to these HLA proteins doesn’t mediate a discount in viral infectivity.

Induction of antibody response within the oral cavity of canines following intraocular (eye drop) immunization with Porphyromonas gingivalis cell lysate integrated in pH-sensitive fusogenic polymer-modified liposomes.
Induction of mucosal immune responses in opposition to Porphyromonas gingivalis inside the oral cavity of canines was studied by immunizing with pH-sensitive fusogenic polymer (MGluPG)-modified liposome-associated cell lysate.
Canines immunized with P. gingivalis cell lysate-containing MGluPG-modified liposomes by intraocular (eye drop) route displayed important ranges of P. gingivalis cell lysate-specific serum IgG and IgA in addition to mucosal IgA antibodies in saliva secretion.
Serum and salivary antibodies generated by intraocularly immunized with MGluPG-modified liposome-associated P. gingivalis cell lysate revealed a big aggregation exercise in opposition to P. gingivalis, whereas serum and saliva from canines receiving MGluPG-modified liposomes unentrapping P. gingivalis cell lysate didn’t present the aggregation exercise in opposition to P. gingivalis.
Moreover, P. gingivalis-specific antibodies in saliva of immunized canines inhibited the adherence of P. gingivalis to cultured HeLa cells. Extra importantly, salivary antibodies induced by intraocular immunization with P. gingivalis cell lysate-containing MGluPG-modified liposomes considerably inhibited the coaggregation of P. gingivalis with Actinomyces naeslundii and the cell harm exercise of P. gingivalis in opposition to FaDu cells, an oral epithelial cell.
These outcomes counsel that intraocularly administered P. gingivalis cell lysate-containing MGluPG-modified liposomes ought to be an efficient mucosal vaccine in opposition to P. gingivalis an infection in canines and could also be an necessary software for the prevention of periodontitis.
Technology of neutralizing monoclonal antibodies in opposition to a conformational epitope of human adenovirus kind 7 (HAdv-7) integrated in capsid encoded in a HAdv-3-based vector.
The technology of monoclonal antibodies (MAbs) by epitope-based immunization is tough as a result of the immunogenicity of straightforward peptides is poor and T cells have to be potently stimulated and immunological reminiscence elicited.
A method through which antigen is integrated into the adenoviral capsid protein has been used beforehand to develop antibody responses in opposition to a number of vaccine targets and should supply an answer to this drawback.
On this research, we used an identical technique to develop HAdv-7-neutralizing MAbs utilizing rAdMHE3 virions into which hexon hypervariable area 5 (HVR5) of adenovirus kind 7 (HAdv-7) was integrated.
The epitope mutant rAdMHE3 was generated by changing HVR5 of Ad3EGFP, a recombinant HAdv-3-based vector expressing enhanced inexperienced fluorescence protein, with HVR5 of HAdv-7.
We immunized BALB/c mice with rAdMHE3 virions and produced 22 completely different MAbs in opposition to them, 4 of which confirmed neutralizing exercise in opposition to HAdv-7 in vitro.
Utilizing an oblique enzyme-linked immunosorbent assay (ELISA) evaluation and an antibody-binding-competition ELISA with Ad3EGFP, HAdv-7, and a collection of chimeric adenoviral particles containing epitope mutants, we demonstrated that the 4 MAbs acknowledge the neutralization web site inside HVR5 of the HAdv-7 virion.
Utilizing an immunoblotting evaluation and ELISA with HAdv-7, recombinant peptides, and an artificial peptide, we additionally confirmed that the neutralizing epitope inside HVR5 of the HAdv-7 virion is a conformational epitope.
Goat Cathepsin Antibodies ELISA kit |
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E06C0695-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Goat Cathepsin Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Cathepsin Antibodies ELISA kit |
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E06C0695-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Goat Cathepsin Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Cathepsin Antibodies ELISA kit |
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E04C0695-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Rabbit Cathepsin Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Cathepsin Antibodies ELISA kit |
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E04C0695-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Rabbit Cathepsin Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Cathepsin Antibodies ELISA kit |
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E04C0695-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Rabbit Cathepsin Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Cathepsin Antibodies ELISA kit |
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E08C0695-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Canine Cathepsin Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Cathepsin Antibodies ELISA kit |
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E08C0695-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Canine Cathepsin Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Cathepsin Antibodies ELISA kit |
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E08C0695-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Canine Cathepsin Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cathepsin Antibodies ELISA kit |
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E09C0695-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Monkey Cathepsin Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cathepsin Antibodies ELISA kit |
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E09C0695-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Monkey Cathepsin Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cathepsin Antibodies ELISA kit |
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E09C0695-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Monkey Cathepsin Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Cathepsin Antibodies ELISA kit |
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E01C0695-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Human Cathepsin Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Cathepsin Antibodies ELISA kit |
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E01C0695-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Human Cathepsin Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Cathepsin Antibodies ELISA kit |
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E01C0695-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Human Cathepsin Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Cathepsin Antibodies ELISA kit |
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E02C0695-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Rat Cathepsin Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Cathepsin Antibodies ELISA kit |
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E02C0695-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Rat Cathepsin Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Cathepsin Antibodies ELISA kit |
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E02C0695-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Rat Cathepsin Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Cathepsin Antibodies ELISA kit |
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E03C0695-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Mouse Cathepsin Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Cathepsin Antibodies ELISA kit |
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E03C0695-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Mouse Cathepsin Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Cathepsin Antibodies ELISA kit |
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E03C0695-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Mouse Cathepsin Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Cathepsin Antibodies ELISA kit |
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E07C0695-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Porcine Cathepsin Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Cathepsin Antibodies ELISA kit |
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E07C0695-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Porcine Cathepsin Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Cathepsin Antibodies ELISA kit |
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E07C0695-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Porcine Cathepsin Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Anti-HMGB1 Peptide (2-11) Antibodies |
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7029 | Chondrex | 1 mg/ml x 0.1 ml | EUR 406.26 |
Description: Anti-HMGB1 Peptide (2-11) Antibodies |
Anti-HMGB1 Peptide (166-176) Antibodies |
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7030 | Chondrex | 1 mg/ml x 0.1 ml | EUR 406.26 |
Description: Anti-HMGB1 Peptide (166-176) Antibodies |
Custom Antibodies to Proteins Order form |
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CAB-F1 | Alpha Diagnostics | 1 | Ask for price |
Goat Platelet antibodies IgG ELISA kit |
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E06P0594-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Goat Platelet antibodies IgG in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Platelet antibodies IgG ELISA kit |
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E06P0594-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Goat Platelet antibodies IgG in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Platelet antibodies IgG ELISA kit |
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E06P0594-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Goat Platelet antibodies IgG in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Platelet antibodies IgA ELISA kit |
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E06P0596-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Goat Platelet antibodies IgA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Platelet antibodies IgA ELISA kit |
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E06P0596-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Goat Platelet antibodies IgA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Platelet antibodies IgA ELISA kit |
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E06P0596-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Goat Platelet antibodies IgA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Anti Apolipoprotein Antibodies ELISA kit |
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E04A0447-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Rabbit Anti Apolipoprotein Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Anti Apolipoprotein Antibodies ELISA kit |
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E04A0447-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Rabbit Anti Apolipoprotein Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Anti Apolipoprotein Antibodies ELISA kit |
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E04A0447-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Rabbit Anti Apolipoprotein Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Anti acrosin antibodies ELISA kit |
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E04A0718-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Rabbit Anti acrosin antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Anti acrosin antibodies ELISA kit |
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E04A0718-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Rabbit Anti acrosin antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Anti acrosin antibodies ELISA kit |
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E04A0718-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Rabbit Anti acrosin antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Guinea pig Cathepsin Antibodies ELISA kit |
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E05C0695-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Guinea pig Cathepsin Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Guinea pig Cathepsin Antibodies ELISA kit |
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E05C0695-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Guinea pig Cathepsin Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Guinea pig Cathepsin Antibodies ELISA kit |
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E05C0695-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Guinea pig Cathepsin Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Platelet antibodies IgG ELISA kit |
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E04P0594-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Rabbit Platelet antibodies IgG in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Platelet antibodies IgG ELISA kit |
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E04P0594-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Rabbit Platelet antibodies IgG in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Platelet antibodies IgG ELISA kit |
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E04P0594-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Rabbit Platelet antibodies IgG in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Platelet antibodies IgA ELISA kit |
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E04P0596-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Rabbit Platelet antibodies IgA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
These findings counsel that it’s possible to make use of a technique through which antigen is integrated into the adenoviral capsid protein to generate neutralizing MAbs. This technique can also be helpful for growing therapeutic neutralizing MAbs and designing recombinant vector vaccines in opposition to HAdv-7, and in structural evaluation of adenoviruses.