Lipid Peroxidation Assay Kit

Lipid peroxidation assay protocol summary:

– add TBA reply to samples and necessities, incubate at 95ºC for 60 min, cool in ice bathtub for 10 min
– swap to wells of microplate
– analyze with microplate reader
For greater sensitivity, precipitate with n-butanol, centrifuge, dry and resuspend pellet sooner than analysis.

Product overview

Lipid Peroxidation (MDA + HNE) Assay Bundle

Lipid peroxidation is a extensively acknowledged occasion of oxidative hurt in cell membranes, lipoproteins, and utterly utterly totally different lipid-containing buildings. Peroxidative modification of unsaturated phospholipids, glycolipids, and ldl ldl ldl ldl cholesterol can occur in quite a few reactions. They’re normally triggered by i) free radical species equal to oxyl radicals, peroxyl radicals, and hydroxyl radicals derived from iron-mediated low value of hydrogen peroxide or ii) non-radical species equal to singlet oxygen, ozone, and peroxynitrite generated by the response of superoxide with nitric oxide.

Malondialdehyde (MDA) and 4-hydroxyalkenals are essential toxic byproducts of lipid peroxidation. So, the measurement of the components of such aldehydes corresponds to an index of lipid peroxidation in vitro and in vivo. 4-Hydroxynonenal (4-HNE) is a necessary product of the peroxidative decomposition of ω-6 polyunsaturated fatty acids (PUFA). It possesses cytotoxic, hepatotoxic, mutagenic, and genotoxic properties. Furthermore, elevated ranges of HNE had been current in plasma and quite a few organs beneath oxidative stress circumstances. The very fact is, MDA is in a great deal of circumstances virtually definitely principally in all probability probably the most ample specific specific individual aldehyde ensuing from lipid peroxidation. In vitro MDA can alter proteins, DNA, RNA, and a substantial amount of utterly utterly totally different biomolecules.

Bioquochem’s LPO assay devices measures MDA and HNE concentrations as an index of lipid peroxidation. Firstly, acid-catalyzed assault on the 3-position of the indole ring initiates the reactions between indoles and aldehydes (MDA and HNE). As a consequence of this, this response offers a diindolylalkane (chromophore) with most absorbance contained within the space of 580-620 nm.

In our assay an indol (Reagent A) reacts quickly with MDA and HNE in acidic medium, yielding a chromophore (C) with a extreme molar extinction coefficient at its maximal absorption wavelength of 586 nm.

• Notes

  Lipid peroxidation refers as soon as extra to the oxidative degradation of lipids. On this course of free radicals take electrons from the lipids (usually in cell membranes), resulting in cell hurt. Quantification of lipid peroxidation is necessary to guage oxidative stress. Lipid peroxidation varieties reactive aldehydes equal to malondialdehyde (MDA) and 4-hydroxynonenal (4- HNE) as pure bi-products. Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) are normally used as markers of lipid peroxidation, and to assay for oxidative hurt / oxidative stress.

  Related merchandise

  Evaluation the oxidative stress marker and assay information, or the overall metabolism assay information to look at extra assays for metabolites, metabolic enzymes, mitochondrial carry out, and oxidative stress, and along with how one can assay metabolic carry out in dwell cells using your plate reader.

  Moreover see the favored 4-HNE Assay Bundle ab238538 instead marker of lipid peroxidation and oxidative stress.

  
  How utterly utterly totally different researchers have used Lipid Peroxidation Assay Bundle ab118970

  The MDA/TBARs assay devices has been utilized in publications in various sample varieties, along with:
  – Human: serum¹, hippocampal principal cell extracts², A375 cultured cell lysates³, plasma and platelet samples⁴
  – Mouse: neuronal cell lysates⁵, coronary coronary coronary coronary heart tissue extract⁶, plasma⁷, cell extracts⁸
  – Rat: hippocampal tissue extracts⁹, cardiomyocyte extracts of cultured cells¹⁰, lung lysates¹¹
  – Pig: serum¹²

  ^{References: 1 – Shen J et al. 2018, 2 – Wang Q et al. 2019, 3 – Luo M et al. 2018,  4 – Mustafa AG et al. 2018, 5 – Murphy Okay et al. 2018, 6 – Guan F et al. 2019, 7 – Costa CRC et al. 2018, 8 – Eleftheriadis T et al. 2019, 9 – Malekiyan et al. 2019, 10 – Zhou Z et al. 2018, 11 – Li L et al. 2018, 12 – Lee SE and Kang KS 2019}

• Platform

  Microplate reader

Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit
K2167-100	ApexBio	100 assays	EUR 703.2

Cell Meterâ„¢ Fluorimetric Cellular Lipid Peroxidation Assay Kit
22906-200Tests	AAT Bioquest	200 Tests	EUR 334
Description: Lipid peroxidation is the oxidative degradation of cellular lipid by reactive oxygen species (ROS).

Cell Meter™ Fluorimetric Cellular Lipid Peroxidation Assay Kit
22906	AAT Bioquest	200 Tests	EUR 334

Cell Meterâ„¢ Intracellular Colorimetric Lipid Peroxidation (MDA) Assay Kit
15991-200tests	AAT Bioquest	200 tests	EUR 446
Description: Lipid peroxidation is characterized by the oxidative degradation of unsaturated fatty acids, phospholipids, glycolipids, cholesterol esters and cholesterol.

Cell Meter™ Intracellular Colorimetric Lipid Peroxidation (MDA) Assay Kit
15991	AAT Bioquest	200 tests	EUR 446

Myeloperoxidase (MPO) Peroxidation Activity Assay Kit
K747-100	Biovision	each	EUR 392.4

OxiSelect Myeloperoxidase Peroxidation Activity Assay Kit (Fluorometric)
STA-805	Cell Biolabs	192 assays	EUR 692.4
Description: Myeloperoxidase (MPO) is a heme-based peroxidase enzyme responsible for antimicrobial activity against a wide range of organisms. It has also been found to be correlated with increased oxidative stress and decreased levels of catalase activity. The OxiSelect Myeloperoxidase Chlorination Activity Assay Kit is a quantitative, fluorometric assay for measuring the myeloperoxidase peroxidation activity within a sample.

Dog Lipid peroxide ELISA kit
E08L0263-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Lipid peroxide ELISA kit
E08L0263-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Lipid peroxide ELISA kit
E08L0263-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Lipid peroxide ELISA kit
E02L0263-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Lipid peroxide ELISA kit
E02L0263-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Lipid peroxide ELISA kit
E02L0263-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Lipid peroxide ELISA kit
E07L0263-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Lipid peroxide ELISA kit
E07L0263-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Lipid peroxide ELISA kit
E07L0263-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Lipid peroxide ELISA kit
E06L0263-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Lipid peroxide ELISA kit
E06L0263-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Goat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Lipid peroxide ELISA kit
E06L0263-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Goat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Lipid peroxide ELISA kit
E03L0263-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Lipid peroxide ELISA kit
E03L0263-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Lipid peroxide ELISA kit
E03L0263-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Lipid peroxide ELISA kit
E01L0263-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Lipid peroxide ELISA kit
E01L0263-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Human Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Lipid peroxide ELISA kit
E01L0263-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Human Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Lipid peroxide ELISA kit
E04L0263-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Lipid peroxide ELISA kit
E04L0263-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Lipid peroxide ELISA kit
E04L0263-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Lipid peroxide ELISA kit
E09L0263-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Monkey Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.