Lipid peroxidation assay protocol summary:
– add TBA reply to samples and necessities, incubate at 95ºC for 60 min, cool in ice bathtub for 10 min
– swap to wells of microplate
– analyze with microplate reader
For greater sensitivity, precipitate with n-butanol, centrifuge, dry and resuspend pellet sooner than analysis.
Product overview
Lipid Peroxidation (MDA) Assay Bundle (Colorimetric/Fluorometric) (ab118970) offers a helpful software program program program for delicate detection of malondialdehyde (MDA).
Contained within the lipid peroxidation assay protocol, the MDA contained within the sample reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct. The MDA-TBA adduct could also be merely quantified colorimetrically (OD = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA ranges as little as 1 nmol/effectively colorimetrically and 0.1 nmol/effectively fluorometrically.
The MDA assay will even be refered to as a TBARS assay.
Lipid Peroxidation (MDA + HNE) Assay Bundle
Lipid peroxidation is a extensively acknowledged occasion of oxidative hurt in cell membranes, lipoproteins, and utterly utterly totally different lipid-containing buildings. Peroxidative modification of unsaturated phospholipids, glycolipids, and ldl ldl ldl ldl cholesterol can occur in quite a few reactions. They’re normally triggered by i) free radical species equal to oxyl radicals, peroxyl radicals, and hydroxyl radicals derived from iron-mediated low value of hydrogen peroxide or ii) non-radical species equal to singlet oxygen, ozone, and peroxynitrite generated by the response of superoxide with nitric oxide.
Malondialdehyde (MDA) and 4-hydroxyalkenals are essential toxic byproducts of lipid peroxidation. So, the measurement of the components of such aldehydes corresponds to an index of lipid peroxidation in vitro and in vivo. 4-Hydroxynonenal (4-HNE) is a necessary product of the peroxidative decomposition of ω-6 polyunsaturated fatty acids (PUFA). It possesses cytotoxic, hepatotoxic, mutagenic, and genotoxic properties. Furthermore, elevated ranges of HNE had been current in plasma and quite a few organs beneath oxidative stress circumstances. The very fact is, MDA is in a great deal of circumstances virtually definitely principally in all probability probably the most ample specific specific individual aldehyde ensuing from lipid peroxidation. In vitro MDA can alter proteins, DNA, RNA, and a substantial amount of utterly utterly totally different biomolecules.
Bioquochem’s LPO assay devices measures MDA and HNE concentrations as an index of lipid peroxidation. Firstly, acid-catalyzed assault on the 3-position of the indole ring initiates the reactions between indoles and aldehydes (MDA and HNE). As a consequence of this, this response offers a diindolylalkane (chromophore) with most absorbance contained within the space of 580-620 nm.
In our assay an indol (Reagent A) reacts quickly with MDA and HNE in acidic medium, yielding a chromophore (C) with a extreme molar extinction coefficient at its maximal absorption wavelength of 586 nm.

Chinese language language language language protocol within the market. See protocols half beneath.
For an alternate MDA assay, with out the heating steps required contained within the TBARS assay, try MDA assay ab233471.
-
Notes
Lipid peroxidation refers as soon as extra to the oxidative degradation of lipids. On this course of free radicals take electrons from the lipids (usually in cell membranes), resulting in cell hurt. Quantification of lipid peroxidation is necessary to guage oxidative stress. Lipid peroxidation varieties reactive aldehydes equal to malondialdehyde (MDA) and 4-hydroxynonenal (4- HNE) as pure bi-products. Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) are normally used as markers of lipid peroxidation, and to assay for oxidative hurt / oxidative stress.
Related merchandise
Evaluation the oxidative stress marker and assay information, or the overall metabolism assay information to look at extra assays for metabolites, metabolic enzymes, mitochondrial carry out, and oxidative stress, and along with how one can assay metabolic carry out in dwell cells using your plate reader.
Moreover see the favored 4-HNE Assay Bundle ab238538 instead marker of lipid peroxidation and oxidative stress.
How utterly utterly totally different researchers have used Lipid Peroxidation Assay Bundle ab118970The MDA/TBARs assay devices has been utilized in publications in various sample varieties, along with:
– Human: serum1, hippocampal principal cell extracts2, A375 cultured cell lysates3, plasma and platelet samples4
– Mouse: neuronal cell lysates5, coronary coronary coronary coronary heart tissue extract6, plasma7, cell extracts8
– Rat: hippocampal tissue extracts9, cardiomyocyte extracts of cultured cells10, lung lysates11
– Pig: serum12References: 1 – Shen J et al. 2018, 2 – Wang Q et al. 2019, 3 – Luo M et al. 2018, 4 – Mustafa AG et al. 2018, 5 – Murphy Okay et al. 2018, 6 – Guan F et al. 2019, 7 – Costa CRC et al. 2018, 8 – Eleftheriadis T et al. 2019, 9 – Malekiyan et al. 2019, 10 – Zhou Z et al. 2018, 11 – Li L et al. 2018, 12 – Lee SE and Kang KS 2019
-
Platform
Microplate reader
Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit |
|||
K739-100 | Biovision | each | EUR 698.4 |
Cell Meter™ Fluorimetric Cellular Lipid Peroxidation Assay Kit |
|||
22906 | AAT Bioquest | 200 Tests | EUR 367.2 |
Cell Meter™ Intracellular Colorimetric Lipid Peroxidation (MDA) Assay Kit |
|||
15991 | AAT Bioquest | 200 tests | EUR 471.6 |
Myeloperoxidase (MPO) Peroxidation Activity Assay Kit |
|||
K747-100 | Biovision | each | EUR 392.4 |
OxiSelect Myeloperoxidase Peroxidation Activity Assay Kit (Fluorometric) |
|||
STA-805 | Cell Biolabs | 192 assays | EUR 692.4 |
Description: Myeloperoxidase (MPO) is a heme-based peroxidase enzyme responsible for antimicrobial activity against a wide range of organisms. It has also been found to be correlated with increased oxidative stress and decreased levels of catalase activity. The OxiSelect Myeloperoxidase Chlorination Activity Assay Kit is a quantitative, fluorometric assay for measuring the myeloperoxidase peroxidation activity within a sample. |
Lipid A Lipopolysaccharide (LPS Lipid A / Endotoxin) Antibody |
|||
abx415762-1ml | Abbexa | 1 ml | EUR 727.2 |
Lipid A Antibody |
|||
abx023083-1ml | Abbexa | 1 ml | EUR 886.8 |
Lipid Extraction & Polar/Neutral Lipid Separation Combo Kit (Chloroform Free) |
|||
MET-5009-C | Cell Biolabs | 50 preps | EUR 790.8 |
Description: This kit combines a chloroform free organic lipid extraction with further separation of polar and neutral lipids. The lipid extraction first extracts lipids from a crude lipid source to a chloroform free upper organic phase using a proprietary alcohol. After addition of a proprietary organic compound, the mixture is centrifuged to separate the phases. The recovered organic phase containing lipids is dried and then polar and neutral lipids are separated by adding a proprietary secondary alcohol and organic compound. The layers are separated, dried, and resuspended for further analysis. |
Ceraplex Lipid Concentrate(1000X) |
|||
C3316-010 | GenDepot | 100ml | EUR 144 |
Ceraplex Lipid Concentrate(1000X) |
|||
C3316-050 | GenDepot | 500ml | EUR 295.2 |
Lipid Droplet Isolation Kit |
|||
MET-5011 | Cell Biolabs | 50 preps | EUR 498 |
Description: Lipid droplets are lipid rich organelles found in the adipose tissue of eukaryotes. They function to regulate the hydrolysis and storage of neutral lipids and serve as storage for cholesterol and acyl-glycerols. Lipid droplets have also been associated with inflammatory responses, obesity, atherosclerosis, and cancer. |
Lipid Quantitation Kit (Colorimetric) |
|||
STA-613 | Cell Biolabs | 100 assays | EUR 637.2 |
Description: Cell Biolabs? Lipid Quantification Kit measures the lipid content (unsaturated fatty acids only) of samples using the sulfo-phospho-vanillin method, resulting in a simple colorimetric readout amenable to multi-well plate detection. |
Lipid Quantification Kit (Fluorometric) |
|||
STA-617 | Cell Biolabs | 100 assays | EUR 637.2 |
Description: The Lipid Quantification Kit (Fluorometric) uses a fluorometric detection system to quantify neutral lipids in a convenient 96-well microplate reader. The kit includes sufficient reagents for 100 assays. |
Anti-Lipid A antibody |
|||
STJ16100142 | St John's Laboratory | 1 mL | EUR 346.8 |
Monophosphoryl Lipid A (Synthetic) |
|||
VAdv-Ly0012-1mg | Creative Biolabs | 1 mg | EUR 1033.2 |
Description: Synthetic Monophosphoryl Lipid A, pure compound, a TLR4-based vaccine adjuvant. |
Monophosphoryl Lipid A (Synthetic) |
|||
VAdv-Ly0012-5mg | Creative Biolabs | 5 mg | EUR 3013.2 |
Description: Synthetic Monophosphoryl Lipid A, pure compound, a TLR4-based vaccine adjuvant. |
Synthetic Monophosphoryl Lipid A |
|||
VAdv-Ly0031 | Creative Biolabs | 1 mg | EUR 2557.2 |
Description: Synthetic monophosphoryl lipid A, a TLR4 agonist vaccine adjuvant. |
MicroGram Lipid Assay Kit |
|||
LIP1000 | ProFoldin | 1000 assays | EUR 263.3 |
Description: This product includes 0.1 ml of 1000 x LIP 10 Dye. It is for measurement of 1000 samples using 96-well plates. Cuvettes may also be used for measurements. |
LipidSpot 488 Lipid Droplet Stain |
|||
70065 | Biotium | 125uL | EUR 481.2 |
Description: Minimum order quantity: 1 unit of 125uL |
LipidSpot 488 Lipid Droplet Stain |
|||
70065-T | Biotium | 20uL | EUR 139.2 |
Description: Minimum order quantity: 1 unit of 20uL |
LipidSpot 610 Lipid Droplet Stain |
|||
70069 | Biotium | 125uL | EUR 481.2 |
Description: Minimum order quantity: 1 unit of 125uL |
LipidSpot 610 Lipid Droplet Stain |
|||
70069-T | Biotium | 20uL | EUR 136.8 |
Description: Minimum order quantity: 1 unit of 20uL |
Serum Albumin, Lipid Free Protein |
|||
20-abx260049 | Abbexa |
|
|
Goat Lipid peroxide ELISA kit |
|||
E06L0263-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Goat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Lipid peroxide ELISA kit |
|||
E06L0263-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Goat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Lipid peroxide ELISA kit |
|||
E06L0263-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Goat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Lipid peroxide ELISA kit |
|||
E04L0263-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Rabbit Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Lipid peroxide ELISA kit |
|||
E04L0263-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Rabbit Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Lipid peroxide ELISA kit |
|||
E04L0263-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Rabbit Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |