Assay principle
The coated well immunoenzymatic assay for the quantitative measurement of analyte utilizes a multiclonal anti‐analyte antibody and an analyte‐HRP conjugate. The assay sample and buffer are incubated together with analyte‐HRP conjugate in pre‐coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme‐substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the
solution yellow.

The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the analyte concentration since analyte from samples and analyte ‐HRP conjugate compete for the anti‐analyte antibody binding site. Since the number of sites is limited, as more sites are occupied by
analyte from the sample, fewer sites are left to bind analyte‐HRP conjugate.

Standards of known analyte concentrations are run concurrently with the samples being assayed and a standard
curve is plotted relating the intensity of the color (Optical Density) to the concentration of analyte. The analyte concentration in each sample is interpolated from this standard curve.

ELISAs and ELISA Kits

 

MicroElute PCR Clean Up Kit (200prep)
FAMPK-001-1	Favorgen	200 preps	EUR 224.4

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered
SFBM31-0100	Equitech	100 ml	EUR 308.88

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered
SFBM31-0500	Equitech	500 ml	EUR 780

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered
SFBU31-0100	Equitech	100 ml	EUR 354.12

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered
SFBU31-0500	Equitech	500 ml	EUR 900.12

Sterile filtered chicken serum, 0.2 micron, charcoal stripped, heat-inactivated
SC3132-0050	Equitech	50 ml	EUR 199.68

Sterile filtered chicken serum, 0.2 micron, charcoal stripped, heat-inactivated
SC3132-0100	Equitech	100 ml	EUR 226.2

Sterile filtered chicken serum, 0.2 micron, charcoal stripped, heat-inactivated
SC3132-0500	Equitech	500 ml	EUR 302.64

MicroElute GEL Purification Kit (50prep)
FAMGK-001	Favorgen	50 preps	EUR 151.2

MicroElute GEL Purification Kit (200prep)
FAMGK-001-1	Favorgen	200 preps	EUR 224.4

MicroElute GEL/PCR Purification Kit (50prep)
FAEPK-001	Favorgen	50 preps	EUR 157.2

MicroElute GEL/PCR Purification Kit (200prep)
FAEPK-001-1	Favorgen	200 preps	EUR 249.6

MicroElute GEL/PCR Purification Kit (300prep)
FAEPK-001-2	Favorgen	300 preps	EUR 274.8

Soil/ Stool RNA Extraction MicroElute Kit (100prep)
FAFRK-001-MICRO	Favorgen	100 preps	EUR 363.6

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered, heat-inactivate
SFBM3132-0100	Equitech	100 ml	EUR 330.72

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered, heat-inactivate
SFBM3132-0500	Equitech	500 ml	EUR 787.8

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered, heat-inactivate
SFBU3132-0100	Equitech	100 ml	EUR 360.36

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered, heat-inactivate
SFBU3132-0500	Equitech	500 ml	EUR 907.92

Block for Microtube Strips (2.0mL) 6 strips
BLO1288	Scientific Laboratory Supplies	EACH	EUR 298.8

Block, for Microtube Strips (2.0ml) 6 strips
BSW2MTS	Benchmark Scientific	1 each	EUR 224

Tissue Genomic DNA Extraction MicroElute Kit (50prep), with Proteinase K Powder
FATGM-001	Favorgen	50 preps	EUR 202.8

Tissue Genomic DNA Extraction MicroElute Kit (100prep), with Proteinase K Powder
FATGM-001-1	Favorgen	100 preps	EUR 276