Predominant mesangial IgM, C3, and λ light chain depositions and interstitial nephritis in a patient with overlap syndrome and positivity for anti-mitochondrial M2 antibody: a case report

Predominant mesangial IgM, C3, and λ light chain depositions and interstitial nephritis in a patient with overlap syndrome and positivity for anti-mitochondrial M2 antibody: a case report
Overlap syndrome refers to a gaggle of situations which have scientific options of a couple of well-characterised rheumatic illness and meet the respective classification standards. There aren’t any typical renal histological findings in overlap syndrome.
When sufferers with overlap syndrome develop renal dysfunction, varied potential causes, together with lupus nephritis (LN), renal disaster by systemic sclerosis, interstitial nephritis, and so forth, should be distinguished.
Right here, we report a 44-year-old girl with overlap syndrome involving systemic lupus erythematosus (SLE), diffuse cutaneous systemic scleroderma, and Sjogren’s syndrome, who was additionally optimistic for anti-mitochondrial M2 antibody.
She developed glomerular haematuria, proteinuria, and improve in creatinine appeared regularly. Suspecting LN, renal biopsy was carried out. Nonetheless, within the interstitium, gentle infiltration of lymphocytes and plasma cells and really partial fibrosis had been noticed.
Immunofluorescence microscopy revealed predominant mesangial immunoglobulin M, C3, and λ gentle chain staining. Total, LN was not identified based mostly on these findings.
Renal dysfunction was normalised by glucocorticoid remedy for Three months. This case suggests the significance of a renal prognosis based mostly on renal pathological findings, particularly in a case of overlap syndrome together with SLE.

Concurrent anti-neutrophil cytoplasmic antibody-associated glomerulonephritis and IgG4-associated tubulointerstitial nephritis with C3 glomerulonephritis: A case report.

IgG4-related illness (IgG4-RD) is a slowly progressing inflammatory illness that may contain a number of organ techniques. There’s appreciable overlap between IgG4-RDs and anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV).
Herein, we current an uncommon case of IgG4-associated tubulointerstitial nephritis (IgG4-TIN) and ANCA-associated glomerulonephritis (ANCA-GN) co-occurring with C3 glomerulonephritis (C3GN).A 72-year-old male was admitted to hospital due to fever and fatigue.
He was identified with elevated serum creatinine and IgG4 ranges, and was optimistic for ANCA.Initially, the pathology supported a prognosis of IgG4-TIN and ANCA-GN; nonetheless, additional examination revealed he additionally had C3GN.The affected person was handled with methylprednisolone and cyclophosphamide and acquired common follow-up care.
After remedy, the affected person not exhibited fever or fatigue and had no issues. The seven-month follow-up confirmed downward tendencies in IgG4 and MPO-ANCA ranges and steady 24-hour urine protein, serum creatinine ranges.
Anti-neutrophil cytoplasmic antibody-associated glomerulonephritis and IgG4-associated tubulointerstitial nephritis with C3glomerulonephritis not often happen concurrently.
Laboratory evaluation and pathology are each wanted to make sure diagnostic accuracy. Nonetheless, on this case, the three illnesses overlapped to such a big extent that reaching a definitive prognosis was significantly difficult. Well timed and correct prognosis is essential for choosing the right remedy course and optimizing affected person final result.

Worth of mixed detection of anti-nuclear antibodyanti-double-stranded DNA antibody and C3, C4 enhances within the scientific prognosis of systemic lupus erythematosus.

Mixed detection of antinuclear antibody (ANA), anti-double-stranded DNA (ds-DNA) antibody and enhances C3 and C4 within the prognosis of systemic lupus erythematosus (SLE) was analyzed. 100 and ninety-four sufferers with SLE admitted to Yantaishan Hospital of Yantai from January 2012 to December 2017 had been chosen as SLE group.
A complete of 106 sufferers with non-SLE rheumatic illness had been chosen as illness management group and 120 wholesome topics as wholesome management group. The ANA and anti-ds-DNA antibodies had been detected by ELISA and complement C3 and C4 had been detected by fee nephelometry.
The sensitivity and specificity of those 4 components had been additionally analyzed for the prognosis of SLE. The sensitivity and specificity of ANA in diagnosing SLE had been 91.75 and 79.65%, respectively; of anti-ds-DNA antibody had been 67.01 and 98.23%, respectively; of complement C3 had been 87.11 and 82.74%, respectively; and of complement C4 had been 88.66 and 77.43%, respectively.
The sensitivity and specificity of ANA and anti-ds-DNA antibody within the prognosis of SLE had been 95.36 and 96.90%, respectively; of C3 and C4 had been 92.78 and 79.20%, respectively; and the sensitivity and specificity of the mixture of all 4 indicators had been 97.42 and 80.97%, respectively.
The mixed prognosis of SLE with ANA, anti-ds-DNA antibody, complement C3 and C4 can play a complementary function within the prognosis and remedy of SLE sufferers, and it’s of nice significance to the prognosis and remedy planning of SLE sufferers.
 Predominant mesangial IgM, C3, and λ light chain depositions and interstitial nephritis in a patient with overlap syndrome and positivity for anti-mitochondrial M2 antibody: a case report

The impact of anti-Anisakis simplex antibody ranges on C3 and C4 complement elements in human sera.

Beforehand, an in vitro impact was noticed on the complement system not solely of the excretory-secretory merchandise but additionally of somatic antigens from L3 Anisakis simplex larvae.
Within the current work the impact of anti-A. simplex particular antibodies on C3 and C4 ranges in human sera was investigated. As much as 309 samples of sera had been examined to find out ranges of C3 and C4 and anti-A. simplex antibodies, together with immunoglobulins IgG, IgM, IgA and IgE.
Important variations had been noticed between ranges of C3 and C4 and all immunoglobulins apart from IgE. Within the case of immunoglobulins, the likelihood that an anti-A. simplex optimistic topic has a C3 deficiency was 3.
eight instances greater than a topic with out particular antibodies. In conclusion, an affiliation between elevated ranges of anti-A. simplex antibodies and C3 and C4 deficiency was demonstrated.

Recombinant adenovirus vectors activate the choice complement pathway, resulting in the binding of human complement protein C3 impartial of anti-ad antibodies.

Recombinant adenoviruses are one of the widespread gene switch vectors utilized in human scientific trials, however it’s also clear that systemic administration of this virus shall be met by host innate and adaptive antiviral immune responses.
One ingredient of innate immunity is the complement system, a gaggle of proteins that has developed to quickly acknowledge overseas microbes and viruses and to clear them from the circulatory system previous to their gaining entry to weak host cells.
Extreme complement activation can provoke or propagate a variety of deleterious inflammatory responses, by launch of potent cytokines and anaphylatoxins and/or by direct mobile toxicity.
These reactions can progress quickly and are components necessary in severe issues, together with the systemic inflammatory response syndrome and the grownup respiratory misery syndrome.

The anti-lipid A monoclonal antibody E5 binds to tough gram-negative micro organism, fixes C3, and facilitates binding of bacterial immune complexes to each erythrocytes and monocytes.

Remedy of sufferers with septic shock utilizing monoclonal antibodies (mAbs) to endotoxin remains to be controversial. Medical trials of E5, one of many mAbs directed towards the lipid A moiety of lipopolysaccharide (LPS), are presently in progress.
The mechanisms of motion of this, and different antibodies below scientific analysis, are, nonetheless, poorly understood. On this examine we examined in vitro the methods by which E5 interacted with Gram-negative micro organism, complement, erythrocytes and monocytes.
By fluorescence-activated cell sorter (FACS) evaluation we confirmed direct, dose-dependent binding of E5 to Escherichia coli (E. coli) and Salmonella minnesota (S. minnesota). Antibody binding to S. minnesota was enhanced by remedy with the beta-lactam antibiotic amoxycillin, however not by remedy with the aminoglycoside gentamicin.
Immune complexes fashioned between E5 and each species of Gram-negative micro organism activated each classical and various complement pathways, however solely within the case of S. minnesota did this facilitate binding to erythrocyte CR1 and monocyte CR3. Bacterial C3b and iC3b fixation by E5 was quantified utilizing particular mAbs.

C3 Antibody / Complement C3

RQ6122 100 ug
EUR 356.15
Description: Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients.

C3 Antibody / Complement C3

RQ4854 100ul
EUR 356.15
Description: C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. [UniProt]

Anti-C3 Monoclonal Antibody (WM1)

CTA-286-100ug 100ug Ask for price
Description: Mouse anti-C3 monoclonal antibody (WM1) for IP.

Anti-C3 Monoclonal Antibody (WM1)

CTA-286-1mg 1mg Ask for price
Description: Mouse anti-C3 monoclonal antibody (WM1) for IP.

Anti-AFP Antibody (monoclonal, C3)

MA1001 100ug/vial
EUR 400.8

Goat Anti-C3 polyclonal antibody

CABT-BL8695 1.0 mg
EUR 670.8

Anti-Complement C3 Polyclonal Antibody

CTA-340-100ug 100ug Ask for price
Description: Rabbit anti-complement C3 polyclonal antibodyfor IHC, IHC-P.

Anti-Complement C3 Polyclonal Antibody

CTA-340-1mg 1mg Ask for price
Description: Rabbit anti-complement C3 polyclonal antibodyfor IHC, IHC-P.

Anti-Complement C3 Monoclonal Antibody

M00168-2 100ug
EUR 476.4
Description: Rabbit Monoclonal Complement C3 Antibody. Validated in IP, WB and tested in Human.

C3 Antibody

49413-100ul 100ul
EUR 399.6

C3 Antibody

49413-50ul 50ul
EUR 286.8

C3 Antibody

49761-100ul 100ul
EUR 399.6

C3 Antibody

49761-50ul 50ul
EUR 286.8

C3 Antibody

1-CSB-PA10599A0Rb
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against C3. Recognizes C3 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200

C3 Antibody

DF13224 100ul
EUR 420

C3 Antibody

1-CSB-PA360475HA01RA
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against C3. Recognizes C3 from Rat. This antibody is Unconjugated. Tested in the following application: ELISA

C3 Antibody

1-CSB-PA365507LA01MO
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against C3. Recognizes C3 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:1000-1:5000

Complement C3 Convertase (C3) Antibody

20-abx270259
  • EUR 560.40
  • EUR 644.40
  • EUR 326.40
  • EUR 978.00
  • EUR 427.20
  • 100 tests
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  • 25 tests
  • 500 tests
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Complement C3 Convertase (C3) Antibody

20-abx102334
  • EUR 427.20
  • EUR 159.60
  • EUR 1195.20
  • EUR 594.00
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  • 100 ug
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  • 1 mg
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Complement C3 Convertase (C3) Antibody

20-abx129535
  • EUR 493.20
  • EUR 159.60
  • EUR 1362.00
  • EUR 661.20
  • EUR 376.80
  • 100 ug
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Anti-CD63 Antibody (Clone NKI/C3)

A1557-100 each
EUR 574.8

Anti-Human C3 Antibody | GC3-80A

GC3-80A 1.0 mg
EUR 252
Description:

Anti-Human C3 Antibody ; GC3-80A ; Immunology Consultants Laboratory

Host: Goat

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Polyclonal

Goat Anti Human C3 Polyclonal Antibody

CPBT-65830GH 1 ml
EUR 514.8

Anti-complement C3 (aa681-693) antibody

STJ72872 100 µg
EUR 312

Anti-complement C3 (aa1580-1592) antibody

STJ72871 100 µg
EUR 430.8

Anti-Complement C3 Monoclonal Antibody (5F9)

CTA-079-100ug 100ug Ask for price
Description: Mouse anti-complement C3 monoclonal antibody (5F9) for WB, ELISA, IHC-P.

Anti-Complement C3 Monoclonal Antibody (5F9)

CTA-079-1mg 1mg Ask for price
Description: Mouse anti-complement C3 monoclonal antibody (5F9) for WB, ELISA, IHC-P.

Anti-Complement C3 Monoclonal Antibody (11H9)

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Description: Mouse anti-complement C3 monoclonal antibody (11H9) for WB, Flow, Flow-IC, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready.

Anti-Complement C3 Monoclonal Antibody (11H9)

CTA-048-1mg 1mg Ask for price
Description: Mouse anti-complement C3 monoclonal antibody (11H9) for WB, Flow, Flow-IC, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready.

Anti-Complement Component C3 Polyclonal Antibody

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Description: Rabbit anti-complement component C3 polyclonal antibodyfor WB, Simple Western, ICC/IF, IHC-P, IP.

Anti-Complement Component C3 Polyclonal Antibody

CTA-342-1mg 1mg Ask for price
Description: Rabbit anti-complement component C3 polyclonal antibodyfor WB, Simple Western, ICC/IF, IHC-P, IP.

Complement C3 Beta Chain (C3) Antibody

abx019059-100ug 100 ug
EUR 410.4

Complement C3 Beta Chain (C3) Antibody

abx019060-100ug 100 ug
EUR 410.4

Complement C3 Convertase (C3) Antibody Pair

20-abx370178
  • EUR 1713.60
  • EUR 1111.20
  • 10 × 96 tests
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Complement C3 Convertase (C3) Antibody Pair

20-abx370709
  • EUR 1946.40
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  • 10 × 96 tests
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Complement C3 Convertase (C3) Antibody (FITC)

20-abx273758
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  • EUR 1395.60
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  • 100 ug
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C3/C3b Antibody

46372-100ul 100ul
EUR 302.4

Complement C3 Convertase (C3) Antibody (Biotin)

20-abx271793
  • EUR 477.60
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  • EUR 1296.00
  • EUR 644.40
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Anti-Mouse C3 Antibody | GC3-90A-Z

GC3-90A-Z 0.1 mg
EUR 284
Description:

Anti-Mouse C3 Antibody ; GC3-90A-Z ; Immunology Consultants Laboratory

Host: Goat

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Polyclonal

Anti-Mouse C3 Antibody | GC3-90F-Z

GC3-90F-Z 0.1 mg
EUR 316
Description:

Anti-Mouse C3 Antibody ; GC3-90F-Z ; Immunology Consultants Laboratory

Host: Goat

Format: FITC Conjugated

Product Type: Primary Antibody

Antibody Clonality: Polyclonal

These observations recommend that E5 might improve bacterial clearance in a number of methods:
(1) by facilitating direct complement fixation;
(2) by facilitating the binding of opsonized micro organism to cells of the mononuclear phagocyte system;
(3) by enabling micro organism to bind to erythrocyte CR1 (CD35), permitting secure carriage within the circulation to the fastened macrophages of the liver and spleen;
(4) by appearing synergistically with beta-lactam antibiotics.

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