The Aviva Systems Biology (Mouse) Prtn3 ELISA Kit (OKEH07123) is based on standard sandwich enzyme-linked immunosorbent assay technology. An antibody specific for Prtn3 was precoated in a 96-well plate (12 x 8-well strips) and blocked. Standards or test samples are added to the wells, incubated, and removed. A biotinylated detector antibody specific for Prtn3 is added, incubated, and washed.
The avidin-peroxidase conjugate is then added, incubated, and the unbound conjugate is washed. An enzymatic reaction occurs by adding TMB substrate that is catalyzed by HRP generating a blue product that changes to yellow after adding the acid stop solution. The density of the yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of the Prtn3 sample captured in the well.
- ELISA Kit Range 0.312 – 20ng / mL
- Recovery of the ELISA kit Average recovery when adding to the matrix = 88.9%
ELISA kit reproducibility Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, medium, and high-level LIPC. Interassay reproducibility was evaluated with 3 samples representing low, medium, and high-level LIPC using 8 replicas on each of the 3 plates.
- Intra-assay CV <8.3%
- Interassay CV <10.2%
Reconstitution and Storage Store as indicated in the product manual.
Sample Type Serum, Plasma, Tissue Homogenates
Sensitivity 0.156 ng/mL
Specificity Natural and recombinant Mouse Myeloblastin
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Description of Target
Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and type I, III, and IV collagen (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters.