Total RNA Purification Kits

RNA purification package
RNA purification package

Software program program:

GenElute Full RNA Purification Instruments is used for the extraction of total RNA:

  • From full blood samples all through the detection of Hepatitis C Virus utilizing a reverse transcriptase-polymerase spiral response (RT-PSR) system.
  • From an in a single day customized of bacterial strains in TSB medium in figuring out the implications of curcumin and silymarin on virulence and carbapenemase genes amongst multidrug-resistant Escherichia coli scientific isolates.

Widespread Description:

Sigma′s GenElute Full RNA Purification Instruments supplies a speedy methodology for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, plasma, serum, micro organism, yeast, fungi, vegetation and viruses. The package deal deal purifies all sizes of RNA, from giant mRNA and ribosomal RNA all one of the simplest ways proper right down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from completely completely different cell parts paying homage to proteins, with out using phenol or chloroform. The purified RNA is of the right integrity, and can be utilized in pretty a few downstream features together with exact time PCR, reverse transcription PCR, Northern blotting, RNase safety and primer extension, and expression array assays.

  • Isolate true total RNA together with siRNA and microRNA
  • Quickly take away contaminating genomic DNA with out using enzymes
  • No phenol or chloroform extractions
  • Purify high-quality RNA in 20 minutes
  • Extract RNA from as little as a single cell
  • Isolate from all forms of specimens

The purification strategies of our Full RNA Purification Kits vary from pure extraction reagents all top-of-the-line methods to spin columns and magnetic beads.

Beginning with high-quality, pure, and intact total RNA is critical to many experiments (e.g. RT-PCR, qRT-PCR, array evaluation, Northern blots, nuclease safety assays, and RNA-sequencing). The desk under will current you the way one can select the precise Invitrogen product to purify total RNA out of your particular pattern type and pattern measurement.

Thermo Scientific GeneJET RNA Purification Instruments is an easy and environment nice system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, micro organism, and yeast.

The package deal deal makes use of a silica-based membrane expertise contained in the form of a helpful spin column, eliminating the necessity for tedious cesium chloride gradients, alcohol precipitation, or poisonous phenol-chloroform extractions.

RNA molecules longer than 200 nucleotides will doubtless be remoted with the GeneJET RNA Purification Instruments in 15 minutes after the lysis step. The high-quality purified RNA can be utilized in numerous downstream features, together with RT-PCR, RT-qPCR, Northern blotting, and completely completely different RNA-based analyses.

RNA purification kit
RNA purification equipment

Highlights:


• Widespread – can be utilized for each cell and tissue samples from numerous sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and microorganism

• Setting-friendly – excessive yields of all the RNA.
• Quick – 15 minutes after lysis step
• Pure – an A260/280 ratio > 1.9
• Useful – spin columns are capped and assembled with assortment tubes

Features:


• Quick extraction of utmost purity RNA relevant for all customary molecular biology procedures, together with:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease safety assay incorporates Proteinase Okay Lysis Buffer Wash Buffer 1 (concentrated) Wash Buffer 2 (concentrated) Water, nuclease-free GeneJET RNA Purification Columns (pre-assembled with Assortment Tubes) Assortment Tubes (2 ml) Assortment Tubes (1.5 ml) Detailed Protocol

GenElute Full RNA Purification Instruments has been used to:

  • Quantitative, real-time RT-PCR for each giant mRNA and small RNA together with miRNA
  • RT-PCR for each giant mRNA and small RNA together with miRNA
  • Expression profiling
  • Subsequent Experience Sequencing for RNA and miRNA
  • miRNA from plasma for discovery
  • microRNA cloning and amplification
  • PCR-based virus detection
  • PCR-based viable micro organism detection
  • Northern blotting
  • RNase safety
  • Primer extension
  • miRNA array
  • RNA-Seq
  • Nanostring
  • Fluidigm
  • Droplet/Digital PCR
  • Isolate total RNA of rat kidney tissues

RNA Purification Kit

abx098095-25rxns 25 rxns
EUR 230
  • Shipped within 5-10 working days.

RNA Purification Kit

MB1006-250preps 250 preps
EUR 397

RNA Purification Kit

MB1006-50preps 50 preps
EUR 166

PureXtract RNA Purification Kit

R6111-050 50 prep
EUR 280

PureXtract RNA Purification Kit

R6111-200 200 prep
EUR 874

Human Total PSA (t-PSA) ELISA Kit

PRB-5049-TOTAL 96 assays
EUR 572

RNA isolater Total RNA Extraction Reagent

R401-01 100 ml
EUR 133

Green RNA Lysis Kit 50

GREENE1-RNA 50pack
EUR 180
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Green RNA Lysis Kit 250

GREENE5-RNA 250pack
EUR 571
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Navy RNA Lysis Kit 50

NAVYE1-RNA 50pack
EUR 198
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Navy RNA Lysis Kit 250

NAVYE5-RNA 250pack
EUR 651
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Pink RNA Lysis Kit 50

PINKE1-RNA 50pack
EUR 178
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Pink RNA Lysis Kit 250

PINKE5-RNA 250pack
EUR 561
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RNA Lysis Kit 50

REDE1-RNA 50pack
EUR 192
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RNA Lysis Kit 250

REDE5-RNA 250pack
EUR 622
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RNA Lysis Kit 50

YELLOWE1-RNA 50pack
EUR 192
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RNA Lysis Kit 250

YELLOWE5-RNA 250pack
EUR 624
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

AfterTri-RNA Purification Mini Kit (200prep)

FAATR-001-1 200 preps
EUR 259

ExoQuick Exosome RNA Column Purification kit

EQ808A-1 20 preps
EUR 402

Total RNA Extraction Kit

K2014005 1 kit
EUR 344
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA Isolation Kit

P-9100 50 samples
EUR 347.25
Description: The best epigenetics products

5E GREEN RNA Lysis Kit 100

GREEN5E100-RNA 100pack
EUR 386
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 1000, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

5E GREEN RNA Lysis Kit 20

GREEN5E20-RNA 20pack
EUR 138
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Green RINO RNA Lysis Kit 50

GREENR1-RNA 50pack
EUR 184
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with beads. RNase free.

Green RINO RNA Lysis Kit 250

GREENR5-RNA 250pack
EUR 587
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with beads. RNase free.

5E Navy RNA Lysis Kit 100

NAVY5E100-RNA 100pack
EUR 453
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

5E Navy RNA Lysis Kit 20

NAVY5E20-RNA 20pack
EUR 153
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Navy RINO RNA Lysis Kit 50

NAVYR1-RNA 50pack
EUR 202
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.

Navy RINO RNA Lysis Kit 250

NAVYR5-RNA 250pack
EUR 666
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.

5E PINK RNA Lysis Kit 100

PINK5E100-RNA 100pack
EUR 380
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

5E PINK RNA Lysis Kit 20

PINK5E20-RNA 20pack
EUR 136
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Pink RINO RNA Lysis Kit 50

PINKR1-RNA 50pack
EUR 181
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Pink RINO RNA Lysis Kit 250

PINKR5-RNA 250pack
EUR 576
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

5E RED RNA Lysis Kit 100

RED5E100-RNA 100pack
EUR 429
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

5E RED RNA Lysis Kit 20

RED5E20-RNA 20pack
EUR 148
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RINO RNA Lysis Kit 50

REDR1-RNA 50pack
EUR 195
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Red RINO RNA Lysis Kit 250

REDR5-RNA 250pack
EUR 638
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RINO RNA Lysis Kit 50

YELLOWR1-RNA 50pack
EUR 199
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RINO RNA Lysis Kit 250

YELLOWR5-RNA 250pack
EUR 656
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

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