Software program program:
GenElute Full RNA Purification Instruments is used for the extraction of total RNA:
- From full blood samples all through the detection of Hepatitis C Virus utilizing a reverse transcriptase-polymerase spiral response (RT-PSR) system.
- From an in a single day customized of bacterial strains in TSB medium in figuring out the implications of curcumin and silymarin on virulence and carbapenemase genes amongst multidrug-resistant Escherichia coli scientific isolates.
Widespread Description:
Sigma′s GenElute Full RNA Purification Instruments supplies a speedy methodology for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, plasma, serum, micro organism, yeast, fungi, vegetation and viruses. The package deal deal purifies all sizes of RNA, from giant mRNA and ribosomal RNA all one of the simplest ways proper right down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from completely completely different cell parts paying homage to proteins, with out using phenol or chloroform. The purified RNA is of the right integrity, and can be utilized in pretty a few downstream features together with exact time PCR, reverse transcription PCR, Northern blotting, RNase safety and primer extension, and expression array assays.
- Isolate true total RNA together with siRNA and microRNA
- Quickly take away contaminating genomic DNA with out using enzymes
- No phenol or chloroform extractions
- Purify high-quality RNA in 20 minutes
- Extract RNA from as little as a single cell
- Isolate from all forms of specimens
The purification strategies of our Full RNA Purification Kits vary from pure extraction reagents all top-of-the-line methods to spin columns and magnetic beads.
Beginning with high-quality, pure, and intact total RNA is critical to many experiments (e.g. RT-PCR, qRT-PCR, array evaluation, Northern blots, nuclease safety assays, and RNA-sequencing). The desk under will current you the way one can select the precise Invitrogen product to purify total RNA out of your particular pattern type and pattern measurement.
Thermo Scientific GeneJET RNA Purification Instruments is an easy and environment nice system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, micro organism, and yeast.
The package deal deal makes use of a silica-based membrane expertise contained in the form of a helpful spin column, eliminating the necessity for tedious cesium chloride gradients, alcohol precipitation, or poisonous phenol-chloroform extractions.
RNA molecules longer than 200 nucleotides will doubtless be remoted with the GeneJET RNA Purification Instruments in 15 minutes after the lysis step. The high-quality purified RNA can be utilized in numerous downstream features, together with RT-PCR, RT-qPCR, Northern blotting, and completely completely different RNA-based analyses.
Highlights:
• Widespread – can be utilized for each cell and tissue samples from numerous sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and microorganism
• Setting-friendly – excessive yields of all the RNA.
• Quick – 15 minutes after lysis step
• Pure – an A260/280 ratio > 1.9
• Useful – spin columns are capped and assembled with assortment tubes
Features:
• Quick extraction of utmost purity RNA relevant for all customary molecular biology procedures, together with:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease safety assay incorporates Proteinase Okay Lysis Buffer Wash Buffer 1 (concentrated) Wash Buffer 2 (concentrated) Water, nuclease-free GeneJET RNA Purification Columns (pre-assembled with Assortment Tubes) Assortment Tubes (2 ml) Assortment Tubes (1.5 ml) Detailed Protocol
GenElute Full RNA Purification Instruments has been used to:
- Quantitative, real-time RT-PCR for each giant mRNA and small RNA together with miRNA
- RT-PCR for each giant mRNA and small RNA together with miRNA
- Expression profiling
- Subsequent Experience Sequencing for RNA and miRNA
- miRNA from plasma for discovery
- microRNA cloning and amplification
- PCR-based virus detection
- PCR-based viable micro organism detection
- Northern blotting
- RNase safety
- Primer extension
- miRNA array
- RNA-Seq
- Nanostring
- Fluidigm
- Droplet/Digital PCR
- Isolate total RNA of rat kidney tissues
 RNA Purification Kit |
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| abx098095-25rxns | Abbexa | 25 rxns | EUR 230 |
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RNA Purification Kit |
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| MB1006-250preps | BosterBio | 250 preps | EUR 397 |
RNA Purification Kit |
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| MB1006-50preps | BosterBio | 50 preps | EUR 166 |
 PureXtract RNA Purification Kit |
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| R6111-050 | GenDepot | 50 prep | EUR 280 |
PureXtract RNA Purification Kit |
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| R6111-200 | GenDepot | 200 prep | EUR 874 |
 ELISA Kit) Human Total PSA (t-PSA) ELISA Kit |
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| PRB-5049-TOTAL | Cell Biolabs | 96 assays | EUR 572 |
 RNA isolater Total RNA Extraction Reagent |
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| R401-01 | Vazyme | 100 ml | EUR 133 |
 Green RNA Lysis Kit 50 |
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| GREENE1-RNA | Next Advance | 50pack | EUR 180 |
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Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free. |
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 Green RNA Lysis Kit 250 |
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| GREENE5-RNA | Next Advance | 250pack | EUR 571 |
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Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free. |
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 Navy RNA Lysis Kit 50 |
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| NAVYE1-RNA | Next Advance | 50pack | EUR 198 |
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Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free. |
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 Navy RNA Lysis Kit 250 |
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| NAVYE5-RNA | Next Advance | 250pack | EUR 651 |
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Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free. |
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 Pink RNA Lysis Kit 50 |
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| PINKE1-RNA | Next Advance | 50pack | EUR 178 |
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Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
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 Pink RNA Lysis Kit 250 |
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| PINKE5-RNA | Next Advance | 250pack | EUR 561 |
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Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
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 Red RNA Lysis Kit 50 |
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| REDE1-RNA | Next Advance | 50pack | EUR 192 |
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Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
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 Red RNA Lysis Kit 250 |
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| REDE5-RNA | Next Advance | 250pack | EUR 622 |
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Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
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 YELLOW RNA Lysis Kit 50 |
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| YELLOWE1-RNA | Next Advance | 50pack | EUR 192 |
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Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
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 YELLOW RNA Lysis Kit 250 |
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| YELLOWE5-RNA | Next Advance | 250pack | EUR 624 |
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Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
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) AfterTri-RNA Purification Mini Kit (200prep) |
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| FAATR-001-1 | Favorgen | 200 preps | EUR 259 |
 ExoQuick Exosome RNA Column Purification kit |
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| EQ808A-1 | SBI | 20 preps | EUR 402 |
 Total RNA Extraction Kit |
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| K2014005 | Biochain | 1 kit | EUR 344 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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 Total RNA Isolation Kit |
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| P-9100 | EpiGentek | 50 samples | EUR 347.25 |
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Description: The best epigenetics products |
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 5E GREEN RNA Lysis Kit 100 |
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| GREEN5E100-RNA | Next Advance | 100pack | EUR 386 |
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Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 1000, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free. |
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 5E GREEN RNA Lysis Kit 20 |
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| GREEN5E20-RNA | Next Advance | 20pack | EUR 138 |
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Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free. |
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 Green RINO RNA Lysis Kit 50 |
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| GREENR1-RNA | Next Advance | 50pack | EUR 184 |
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Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with beads. RNase free. |
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 Green RINO RNA Lysis Kit 250 |
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| GREENR5-RNA | Next Advance | 250pack | EUR 587 |
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Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with beads. RNase free. |
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 5E Navy RNA Lysis Kit 100 |
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| NAVY5E100-RNA | Next Advance | 100pack | EUR 453 |
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Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free. |
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 5E Navy RNA Lysis Kit 20 |
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| NAVY5E20-RNA | Next Advance | 20pack | EUR 153 |
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Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free. |
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 Navy RINO RNA Lysis Kit 50 |
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| NAVYR1-RNA | Next Advance | 50pack | EUR 202 |
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Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free. |
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 Navy RINO RNA Lysis Kit 250 |
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| NAVYR5-RNA | Next Advance | 250pack | EUR 666 |
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Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free. |
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 5E PINK RNA Lysis Kit 100 |
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| PINK5E100-RNA | Next Advance | 100pack | EUR 380 |
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Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
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 5E PINK RNA Lysis Kit 20 |
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| PINK5E20-RNA | Next Advance | 20pack | EUR 136 |
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Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
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 Pink RINO RNA Lysis Kit 50 |
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| PINKR1-RNA | Next Advance | 50pack | EUR 181 |
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Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free. |
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 Pink RINO RNA Lysis Kit 250 |
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| PINKR5-RNA | Next Advance | 250pack | EUR 576 |
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Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free. |
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 5E RED RNA Lysis Kit 100 |
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| RED5E100-RNA | Next Advance | 100pack | EUR 429 |
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Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
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 5E RED RNA Lysis Kit 20 |
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| RED5E20-RNA | Next Advance | 20pack | EUR 148 |
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Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
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 Red RINO RNA Lysis Kit 50 |
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| REDR1-RNA | Next Advance | 50pack | EUR 195 |
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Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free. |
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 Red RINO RNA Lysis Kit 250 |
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| REDR5-RNA | Next Advance | 250pack | EUR 638 |
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Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free. |
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 YELLOW RINO RNA Lysis Kit 50 |
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| YELLOWR1-RNA | Next Advance | 50pack | EUR 199 |
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Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free. |
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 YELLOW RINO RNA Lysis Kit 250 |
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| YELLOWR5-RNA | Next Advance | 250pack | EUR 656 |
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Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free. |
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