Nationwide Diagnostics ProtoGel kinds an electrophoresis matrix that’s excellent for the separation of proteins and polypeptides. ProtoGel is a stabilized, ready-to-use 30% (w/v) acrylamide/methylene bisacrylamide resolution (37.5:1 ratio) manufactured from the very best high quality supplies from which nearly all impurities have been eliminated. ProtoGel has zero acrylic acid content material, eliminating the mounted costs that trigger band streaking. Moreover, oxidation merchandise similar to aldehydes have been eliminated by a selective adsorption course of. Aldehydes can assault proteins, altering the construction of the pattern, and thus altering Rf values. With ProtoGel, you possibly can belief that your outcomes can be constant one electrophoretic run to the following.
Storage: ProtoGel is steady for 24 months when saved tightly capped in a darkish space at room temperature (20°C).
Protocol:
ProtoGel (30%) Protocol
Security Overview:
ProtoGel (30%) Security Overview
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ProtoGel (30%); Nationwide Diagnostics; 37.5:1 acrylamide to bisacrylamide stabilized soln.; Optimized for SDS-PAGE of proteins (Laemmli gels); Persistently crystal clear gels, zero fluorescence; Stabilized for lengthy shelf life; Measurement: 1L
The usage of Nationwide Diagnostics’ ProtoGel Resolving Buffer and ProtoGel Stacking Buffer will make sure the purity and efficiency of your Laemmli gels. ProtoGel Buffer kinds gels of 0.375 M Tris-HCl and 0.1% SDS, pH 8.8. ProtoGel Stacking Buffer kinds gels of 0.125 M Tris-HCl and 0.1% SDS, pH 6.8.
extremely pure protogel nationwide diagnostics
ProtoGel Resolving Buffer and ProtoGel Stacking Buffer are steady for 24 months when saved tightly capped in a darkish space at room temperature.
